S. Aureus DNA Gyrase

Cat. No.: S418772

Descripción general

Product Info

DNA gyrase subunits were cloned overexpressed and purified using the proprietary company methods.  A single band on SDS-PAGE was detected by CB staining for each subunit.  Cross contamination by topo I was assessed by assaying for relaxation of supercoiled DNA under conditions optimized for type I activity.  Under these conditions, after 2 hours of incubation with supercoiled plasmid DNA, no relaxation products were detectable.

Description

Highly purified S. aureus DNA gyrase is a type II topoisomerase encoded by two genes GyrA and GyrB from staphylococcus aureus.  DNA gyrase is an essential topoisomerase that supercoils DNA through a process of strand breakage/resealing and DNA wrapping.  As a type II enzyme, gyrase is unique in its ability to negatively supercoil a relaxed plasmid DNA substrate.  S. aureus DNA gyrase is also the target for quinolone-based antibacterial agents which act by subverting the enzyme into a DNA damaging agent.  Aladdin offers purified DNA gyrase from the gram positive bacterium, Staphylococcus aureus for use in all aspects of drug development and screening assays.  The enzyme is very active in decatenating and supercoiling KDNA but will also negatively supercoil relaxed  plasmid DNA.  The S. aureus enzyme is a heterotetramer  of GyrA2GyrB2 and is purified as His tagged subunits that are reassembled to make active enzyme.

Shipping&storage

This enzyme is shipped on dry ice and should be stored at -70°C. It is stable undiluted for at least 6 months in this concentrated state. The enzyme can be aliquoted on first thawing to minimize damage from multiple freeze thaw cycles.

Specifications

Product Name

S. Aureus DNA Gyrase

Tipo de molécula

Small molecule

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -80°C,Avoid repeated freezing and thawing

Enviado en

Dry ice packs + Cold packs