Shrimp Hemocyanin (SHC) is a highly soluble hemocyanin derived from shrimp. It can be used as an alternative to Keyhole Limpet Hemocyanin (KLH) as a carrier protein for conjugation with low-molecular-weight molecules such as peptides, nucleic acids, drugs or toxins to render them highly immunogenic. Tests have shown that SHC exhibits immunogenicity comparable to KLH, while its solubility (especially after conjugation with haptens) is significantly higher than that of KLH, allowing greater flexibility in immunogen preparation protocols.Product Format: Lyophilized powder dissolved in PBS. Reconstitute with ultrapure water before use.
Product Features
High Immunogenicity: Perfect replacement for KLH;
High Conjugation Yield: Conjugation efficiency identical to KLH;
High Solubility: Significantly higher solubility than KLH, compatible with uncommon or poorly soluble haptens;
High Purity: Purity > 98%;
Excellent Performance: Validated in hundreds of antibody preparation cases;
Cost-Effective: Price much lower than KLH.
Usage
Conjugation with Hapten
1. Reconstitute lyophilized SHC (SMCC-activated) with an appropriate volume of ultrapure water to a concentration of 10 mg/mL;
2. Dissolve 20 mg of thiol-containing hapten in 5 mL of conjugation buffer (83 mM sodium phosphate, 0.1 M EDTA, 0.9 M NaCl, 0.1 mM TCEP, pH 7.2);
Note: 0.1 mM TCEP is not essential. Its main function is to reduce disulfide bonds in the hapten to thiol groups.
3. Immediately mix the hapten solution with activated SHC at a mass ratio of 1:0.6. React for 2 h at room temperature or overnight at 4 °C;
Note: This conjugation ratio is for reference only. The size and structure of different haptens may affect conjugation efficiency. To ensure efficient conjugation, it is necessary to optimize the hapten-to-SHC ratio via several preliminary experiments according to specific conditions.
3. Remove EDTA by size-exclusion chromatography.
Notes
1. This product is accurately quantified. Please dissolve it directly in the original vial at the desired concentration. DO NOT aliquot the product, as significant loss may occur due to product characteristics / static electricity.
2. The predicted extinction coefficient ε1% of SHC is 10. Adjust the extinction coefficient when determining the concentration of SHC solution.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.
4. For research use only.
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