Singlet Oxygen Sensor Green Reagent

Cat. No.: S750650

Storage

Protected from light,Store at -80°C

Shipped In

Dry ice packs + Cold packs

Descripción general

The SOSG (Singlet Oxygen Sensor Green Fluorescent Probe) produced by Aladdin, also known as Singlet Oxygen Sensor Green, Singlet Oxygen Sensor Green Reagent, or Singlet Oxygen Sensing Green Fluorescent Probe, is a highly selective green fluorescent probe for singlet oxygen (1O2) but lacks cell membrane permeability. It is commonly used for detecting singlet oxygen in solution systems and evaluating the efficacy of free radical scavengers. SOSG singlet oxygen fluorescent probe has high selectivity for 1O2; Unlike other fluorescent or chemiluminescence singlet oxygen detection reagents, it has no significant response to hydroxyl radicals (• OH) and superoxide ion radicals (• O2-). This new singlet oxygen indicator has weak blue fluorescence, with excitation peaks at 372 and 393nm and emission peaks at 395 and 416nm. When singlet oxygen is present, the probe can emit green fluorescence similar to fluorescein (Ex/Im: 504/525nm). The SOSG singlet oxygen fluorescent probe reagent provides a non cell permeable derivative.
The SOSG singlet oxygen fluorescence probe can be used for the detection of 1O2 in aqueous solutions and can also be used to evaluate the efficacy of free radical scavengers, which are commonly used to improve the flavor and nutritional quality of food. It should be noted that the SOSG singlet oxygen fluorescent probe will undergo activation under alkaline pH conditions or in certain solvents, including but not limited to acetonitrile, DMSO, DMF, acetone, etc. Over time, its fluorescent products may also degrade in certain solutions, but if the conditions are properly controlled, the intensity of the green fluorescence signal can be correlated with the concentration of 1O2 without interference from other reactive oxygen species.
Precautions：
1.Fluorescent dyes are prone to quenching, so please avoid exposure to light to minimize fluorescence quenching.We recommend using this product in a sterile environment to ensure stable and consistent assay results.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
2.After receiving the product, immediately dry it and store it in a refrigerator at ≤ -20°C away from light; If long-term storage is required, it is recommended to store in a -80℃ refrigerator. Remove it before use.
Instructions for Use：
1.Preparation of SOSG Stock Solution in methanol: Dissolve 100µg of this product in 33µl of methanol to make a stock solution of approximately 5mM . Note: Aliquot and store the SOSG Stock Solution at -80ºC, and use within one month.
2.Preparation of SOSG Working Solution: The SOSG Working Solution should be prepared immediately before use in aqueous solution, such as ultrapure water or 100mM Tris (pH 7.5). The optimal working concentrations vary depending on experiments, with a suggested starting concentration range of 1-10µM.
3.Any excess SOSG working solution should be discarded, and can not be kept for later use.

Specifications

Estabilidad y almacenamiento

Store at -20°C for 12 months. Store at -80°C for 36 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Condiciones de almacenamiento de almacenamiento

Protected from light, Store at -80°C

Enviado en

Dry ice packs + Cold packs

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Referencias

1. Xianjiao Tang, Xiaojing Yang, Yuhan Wang, Liping Chen, Pengwei Chen, Mei-Xia Zhao.  (2025)  Lysosomal-targeted near-infrared phototherapy agent with type-I photodynamic activity for high-performance synergistic therapy under hypoxia.  Surfaces and Interfaces,      [PMID:] [10.1016/j.surfin.2025.106075]

2. Mingdi Yang, Kaiwen Li, Liangchen Zhong, Yingcui Bu, Yingyong Ni, Ting Wang, Jing Huang, Jingyan Zhang, Hongping Zhou.  (2024)  Molecular engineering to elevate reactive oxygen species generation for synergetic damage on lipid droplets and mitochondria.  ANALYTICA CHIMICA ACTA,      [PMID:38816163] [10.1016/j.aca.2024.342734]

3. Shanshan Jin, Yongkang Chen, Fahui Li, Ping Yan, Guanhong Guo, Guangzhao Xu, Weiguo Song, Wenda Zhong.  (2025)  Photodynamic and Photothermal Co-Induced Efficient Anti-Tumor Immunotherapy.  ACS Applied Materials & Interfaces,      [PMID:40152855] [10.1021/acsami.5c00184]

4. Xiao Zhou, Li Tian, Haotian Wu, Xiying Chen, Jingjing Zhang, Weiran Li, Haijuan Qin, Zhanhui Tao, Shuo Wang, Yaqing Liu.  (2024)  Reusable and self-sterilization mask for real-time personal protection based on sunlight-driven photocatalytic reaction.  JOURNAL OF HAZARDOUS MATERIALS,      [PMID:38262319] [10.1016/j.jhazmat.2024.133513]