SIRP alpha/CD172a Rat mAb (Biotin) - Primary antibody, specific to SIRP alpha/CD172a, Rat IgG1

Cat. No.: Ab191788

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG1

Clone Number

P84

Conjugation

Biotin

Descripción general

Rat anti Mouse SIRP alpha/CD172a Antibody, Monoclonal (P84), could be used for in vivo SIRPα blocking, in vitro SIRPα blocking, WB, IP, Flow and so on.

Specifications

Product Name

SIRP alpha/CD172a Rat mAb (Biotin) - Primary antibody, specific to SIRP alpha/CD172a, Rat IgG1

Sinónimos

Bit | SIRP | SHP-1 | Ptpns1 | Idd13.2 | BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs | Brain Ig-like molecule with tyrosine-based activation motifs | CD172 antigen-like family member A | CD172a antigen | CD172a | Inhibitory

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial

Especies hospedadoras

Rat

Especificidad

SIRP alpha/CD172a

Inmunógeno

Mouse brain membrane protein

Control positivo

Flow: C57BL/6 mouse bone marrow cells.

Conjugación

Biotin

Grado

ExactAb™, Validated

Isotipo

Rat IgG1

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788)

SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788) - Flow Cytometry
Flow cytometry analysis of SIRP alpha/CD172a expression by C57BL/6 mouse bone marrow cells (right panel) compared to an isotype control (left panel). Cells were stained with SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788)

SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788) - Flow Cytometry
Flow cytometry analysis of SIRP alpha/CD172a expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were stained with SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

Objetivos asociados (humanos)

SIRPA Tbio Tyrosine-protein phosphatase non-receptor type substrate 1 (0 Activities)

Discover Target: SIRPA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)