Stabilizers for AP conjugates - STAP

Cat. No.: S491404

GRADE & PURITY STAP

Storage

Store at 2-8°C

Shipped In

Wet ice

Descripción general

1、Product attribute

Shelf life: 36 months

pH Range: 5.8-6.4

Preservative: CMIT/MIT

Boiling point : 100℃

Appearance: colourless - pale yellow liquid

Density (20℃) : 1.0312 g/cm³  

Water solubility: easily soluble

Odour: odourless

Heat sensitive

2、Requirements for storage rooms and vessels

1.Keep container tightly closed.

2.Keep cool.

3.Do not store together with: Oxidizing agent.

4. It is possible to transport the solution at ambient temperature, unless it does not exceed 30 °C. The transport should take less than one week.

3、Effective Components and Principle of Function         

It is based on aqueous buffer system with bovine serum albumin and further protein stabilizing additives, especially balanced to safe the activity of the Alkaline Phosphatase. It has a pH-value of 6.0.

The stabilizer solution provides optimum ambient conditions needed to safe the conformation and enzymatic activity of enzyme conjugates of the Alkaline Phosphatase with antibodies or other proteins. Depending from the pH-sensitivity of the linked protein, it may be advantageous.

4、Biosafety information

The solutions contain biocides for protection from microbiological spoilage.

This mixture is not classified as hazardous in accordance with Regulation (EC) No 1272/2008；

It contains reaction mass of 5-chloro-2-methyl-2H-isothiazol-3-one and 2-methyl-2H-isothiazol-3-one (3:1). May produce an allergic reaction.

5、Advantage

1. Stabilizer for very low concentrated AP conjugates

2. Suitable for proteins resistant to pH < values < 6.0

6、Instruction for usage

1. Any handling to fill in other vessels or splitting has to be done under sterile conditions.

2. Frozen solutions have to be mixed thoroughly after thawing and can be used without restriction afterwards.

3.Solutions showing turbidity should not be used. Since this might be a sign of microbial contamination.

4. Only qualified staff, who are familiar with the production of immunological tests, is permitted to handle this product.

5. The solutions contain bovine serum albumin. This may result in slight shifts of the product properties. Therefore it is recommend to check every new lot for its application in the planned test systems. A reservation of distinct lots is possible.

6. The stabilizer solution is applied undiluted. The biomolecules should be diluted stepwise in the stabilizer solution, depending on the initial concentration and the planned final concentration. Depending on the requirements of the test system concentrations between 50 ng/ml and more than 5 µg/ml are suitable.

7. Enzyme conjugate solution on the basis of this product is suitable for both ELISA and membrane based assay

8. To suppress undesirable effects it is possible to add e.g. HAMA binder and / or further species specific proteins to the solutions.

9. For identification purposes it is possible to add dyes. In case of a request, please contact us.

Specifications

Especificaciones y pureza

STAP

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Nombres e identificadores

• PH: 5.8-6.4

Propiedades químicas y físicas

• Solubilidad: Easily soluble in water
• Sensibilidad: Heat sensitive