Sulfo-NHS-Biotin - ≥85% , CAS No.119616-38-5

CAS: 119616-38-5 Cat. No.: S133214 Peso molecular: 443.428 Número EC: 621-097-1 PubChem CID: 23667738

GRADE & PURITY ≥85%

Synonyms

J-004164 | sodium;1-[5-[(3aS,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate | AKOS030241341 | Sodium;2,5-dioxo-1-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoyloxy]pyrrolid

Storage

Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Application

Proteomics

Descripción general

Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.
Water soluble biotinylation reagent. Typically coupled to primary amine in the pH range 6.5-8.5. Fluorogenic reagent for specific C-terminal labeling of peptides and proteins by carboxypeptidase Y catalyzed transpeptidation reactions.

Specifications

Sinónimos

J-004164 | sodium;1-[5-[(3aS, 6aR)-2-oxo-1, 3, 3a, 4, 6, 6a-hexahydrothieno[3, 4-d]imidazol-4-yl]pentanoyloxy]-2, 5-dioxopyrrolidine-3-sulfonate | AKOS030241341 | Sodium;2, 5-dioxo-1-[5-(2-oxo-1, 3, 3a, 4, 6, 6a-hexahydrothieno[3, 4-d]imidazol-4-yl)pentanoyloxy]pyrrolid

Especificaciones y pureza

≥85%

Mecanismos bioquímicos y fisiológicos

Membrane impermeable, primary amine reactive, short chain (13.5 angstroms) NHS-ester biotinylation reagent. Targets cell surface proteins. Forms irreversible amide bonds. Biotinylates antibodies.

Condiciones de almacenamiento de almacenamiento

Store at -20°C, Argon charged

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Nota

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Pureza

≥85%

Nombres e identificadores

• Pubchem Sid: 504769547
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504769547
• Sonrisas canónicas: C1C(C(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]
• IUPAC Name: sodium;1-[5-[(3aS,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate
• InChIKey: DREOJRVDBCALEG-MJKYAOJXSA-M
• INCHI: 1S/C14H19N3O8S2.Na/c18-10-5-9(27(22,23)24)13(20)17(10)25-11(19)4-2-1-3-8-12-7(6-26-8)15-14(21)16-12;/h7-9,12H,1-6H2,(H2,15,16,21)(H,22,23,24);/q;+1/p-1/t7-,8?,9?,12-;/m0./s1
• Isómeros SMILES: C1[C@H]2[C@@H](C(S1)CCCCC(=O)ON3C(=O)CC(C3=O)S(=O)(=O)[O-])NC(=O)N2.[Na+]
• PubChem CID: 23667738
• Peso molecular: 443.428

Propiedades químicas y físicas

• Solubilidad: Soluble in water (10 mg/ml), and DMF (50 mg/ml)., Soluble in water (10 mg/ml), and DMF (50 mg/ml).

Referencias

1. Tao Peng, Jianyi Wang, Sijun Zhao, Yuyang Zeng, Pimiao Zheng, Demei Liang, Ghulam Mujtaba Mari, Haiyang Jiang.  (2018)  Highly luminescent green-emitting Au nanocluster-based multiplex lateral flow immunoassay for ultrasensitive detection of clenbuterol and ractopamine.  ANALYTICA CHIMICA ACTA,      [PMID:30327104] [10.1016/j.aca.2018.08.014]

2. Peng Tao, Wang Jianyi, Zhao Sijun, Xie Sanlei, Yao Kai, Zheng Pimiao, Wang Sihan, Ke Yuebin, Jiang Haiyang.  (2018)  A fluorometric clenbuterol immunoassay based on the use of organic/inorganic hybrid nanoflowers modified with gold nanoclusters and artificial antigen.  MICROCHIMICA ACTA,  185  (8): (1-8).  [PMID:29982940] [10.1007/s00604-018-2889-0]

3. Lin Luo, Hong-Tao Lei, Jin-Yi Yang, Gong-Liang Liu, Yuan-Ming Sun, Wei-Dong Bai, Hong Wang, Yu-Dong Shen, Sui Chen, Zhen-Lin Xu.  (2016)  Development of an indirect ELISA for the determination of ethyl carbamate in Chinese rice wine.  ANALYTICA CHIMICA ACTA,      [PMID:27916121] [10.1016/j.aca.2016.11.008]

4. Yun-Long Chen, Chen-Chen Zheng, Fu-Jia Tian, Tai-Lang Yin, Xing-Hua Zhang.  (2025)  Stretching a single sugar chain.  CARBOHYDRATE POLYMERS,      [PMID:40973284] [10.1016/j.carbpol.2025.124268]