Supersensitive TMB Chromogen Solution for ELISA

Cat. No.: S743368

Descripción general

Precautions：Aladdin 's series of TMB ELISA substrates have the characteristics of low background, stability, small batch difference, and optional sensitivity. They provide superior signal reaction systems and are suitable for the development and production applications of ELISA kits. The one-component TMB ELISA substrate SS is a hypersensitive TMB substrate, which has the highest sensitivity in this series of TMB ELISA substrates, and has the characteristics of low background and stability. It is suitable for the detection experiment with color development time of 2-10 minutes, and is recommended for qualitative detection.

Instructions for Use：

Application: ELISA.
This product should be balanced to the reaction temperature before use. 
Coloration steps : 
450nm reading :
1.Wash the plate, after HRP enzyme-labeled incubation, wash the plate with PBST / TBST washing buffer ( containing surfactant ) three times.
2.Color, add 100μL substrate, incubate at 20 ~ 37 °C for a certain time, the color is blue.
3.Termination : 100μL TMB termination solution 450 was added, and the color turned yellow.
4.Reading : It is recommended to read the absorbance value at the wavelength of 450 within 30 minutes.
650nm reading : 
1.Wash the plate, after HRP enzyme-labeled incubation, wash the plate with PBST / TBST washing buffer ( including surfactant ) three times.
2.Color development, add 100μL substrate, incubate at 20 ~ 37 °C for a certain time, the color is blue.
3.Termination : 100μL TMB termination solution 650 was added, and the color was blue.
4.Reading : It is recommended to read the absorbance value at the wavelength of 650 within 30 minutes.
kinetics 650nm reading : 
1.Wash the plate, after HRP enzyme-labeled incubation, wash the plate with PBST / TBST washing buffer ( including surfactant ) three times.
2.Color development, add 100μL substrate, incubate at 20 ~ 37 °C.
3.Reading : according to the established frequency ( such as every 1 minute ) read the absorption value at the wavelength of 650 nm.

Specifications

Product Name

Supersensitive TMB Chromogen Solution for ELISA

Tipo de molécula

Unknown

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Estabilidad y almacenamiento

Store at 4℃ in the dark for up to one year.