SYBR Green I Gel Staining Solution, 10000x - 10000 x in DMSO , CAS No.163795-75-3

CAS: 163795-75-3 Cat. No.: S171397 Peso molecular: 509.7 Número EC: 838-836-0

GRADE & PURITY 10000 x in DMSO

Synonyms

2-{[3-(dimethylamino)propyl](propyl)amino}-4-[(E)-(3-methyl-1,3-benzothiazol-2(3H)-ylidene)methyl]-1-phenylquinolinium | 2-{(E)-[2-{[3-(dimethylamino)propyl](propyl)amino}-1-phenylquinolin-4(1H)-ylidene]methyl}-3-methyl-1,3-benzothiazol-3-ium | N,N-dimeth

Storage

Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Descripción general

Sybr Green I and Sybr Green II Nucleic Acid Stains are produced by Dr.Chimin Du, are a kind of novel generation of fluorescent nucleic acid gel stains designed to replace the highly toxic ethidium bromide (EtBr). Sybr Green I is nontoxic and more sensitive than EtBr. Gels can be visualized under UV or Visible Light.

Features of Sybre Green Nucleic Acid Stains
1.  Safety: Sybr Green is nontoxic and noncarcinogenic.
2.  Ultra-sensitivity: It allows the visualization of as little as 20pg dsDNA, around 5-10 times more sensitive than EtBr under
UV and 8-20 times more sensitive than EtBr in Visible Light.
3.  Convenience: NO need to rinse or wash gels. Add stain before load samples. Visualize gels under UV or Visible Light to avoid UV damage on DNA/RNA.
4.  Wide range:   suitable for agrose gel or PAGE.
5.  No impact for the next experiments such as RT, PCR, enzyme digestion, and ligation.
6.  Strong signal and no background
Directions for the Agrose Gels stained by Sybr Green
Protocol 1: Pre-cast Protocol (Add dye in the gel)
1.1 Prepare molten agarose gel solution using your standard protocol.
1.2 Add 1~3µl Sybr Green Nucleic Acid Stain per 50ml gel when the gel cool down to 50℃and mix thoroughly.
1.3 Cast the gel and allow it to solidify. Any leftover gel solution may be stored and reheated later for additional gel casting.
1.4 Load samples and run the gels using your standard protocol.
1.5 DNA stained with SYBR Green I stain can be readily visualized using a UV or blue-light sources (emit at 450, 473, 488, or 532 nm). Image the stained gel with the transilluminator and photograph the gel using.
Protocol 2: Post-staining Protocol (Stain Nucleic Acid after electrophoresis by adding dye in the gel stain solution)
2.1 Make gels: Do not add any nucleic acid stain when make gels.
2.2 Run gels as usual according to your standard protocol.
2.3 Prepare Sybr Green Nucleic Acid Staining solution: Dilute Sybr Green Nucleic Acid Stain with TAE or TBE (TBE (89 mM Tris base, 89 mM
boric acid, 1 mM EDTA, pH 8) and TAE (40 mM Trisacetate,1 mM EDTA, pH 8) on ratio 1:10000. Stain gels in the dark for 10-30min. Staining time depends on gel concentration and thickness. PAGE can be stained directly on the glass. Let staining solution cover PAGE gels for
30min. please use glassware to store staining solution or silicified glassware because stain will absorb on the glass.
2.4 Visualize gels a UV or blue-light sources.
2.5 Exact molecular weight can be measured by this method but the dyes are used much more in this way.
Protocol 3: Stain nucleic acid before electrophoresis (add dye in the loading buffer)
3.1.   Prepare working solution: Dilute 10 µl Sybr Green Stain with 1ml running buffer TBE or TAE. This solution is stable up to one month at 4℃
3.2.   Make gels: based on the routine method. Do not add any DNA/RNA stain in the gel.
3.3.   Stain Nucleic Acid: Add 1µl Sybr Green I Nucleic Acid Stain working solution to 10µ mixture of sample and loading buffer, let it stay at RT for
3-5min for stain binding to nucleic acid completely. Normally, 1µL working solution is enough for one sample loading, and 1ml Sybr Green I Nucleic Acid Stain is enough to load 10,000 samples.
3.4.   Stain markers: Mix 5µL Marker and 1µL Sybr Green I Nucleic Acid Stain working solution thoroughly, let it stay at RT for 5min to let Sybr
Green I Stain and DNA/RNA binding completely.
3.5.   Load samples and run gels.
3.6.   Visualize gels in UV or Visible Light to avoid UV damage on DNA/RNA.
*The big DNA fragments (>2Kb) will move slowly when bind to the stain. So please stain in DNA after electrophoresis or add stain in gels to measure molecular weight exactly.
Notes :
1.  Do not run gels over 2 hrs. Or smeared bands appeared because Sybr Green I Stain will dissociate from DNA/RNA.
2.  Sybr Green I Stain can dissociate from nucleic acids in ethanol.
3.  Please stain nucleic acid in the gel or after electrophoresis to check the exact molecular weight of fragments when compared with molecular weight markers.
4.  Please use EP tubes and other plastic wares in Sybr Green I Stain storage, dilution, and staining. Sybr Green I Stain can bind to glassware.

Specifications

Sinónimos

2-{[3-(dimethylamino)propyl](propyl)amino}-4-[(E)-(3-methyl-1, 3-benzothiazol-2(3H)-ylidene)methyl]-1-phenylquinolinium | 2-{(E)-[2-{[3-(dimethylamino)propyl](propyl)amino}-1-phenylquinolin-4(1H)-ylidene]methyl}-3-methyl-1, 3-benzothiazol-3-ium | N, N-dimeth

Especificaciones y pureza

10000 x in DMSO

Condiciones de almacenamiento de almacenamiento

Store at -20°C, Argon charged

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Nombres e identificadores

• Pubchem Sid: 504765463
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504765463
• Sonrisas canónicas: CCCN(CCCN(C)C)C1=[N+](C2=CC=CC=C2C(=C1)C=C3N(C4=CC=CC=C4S3)C)C5=CC=CC=C5
• IUPAC Name: N,N-dimethyl-N'-[4-[(E)-(3-methyl-1,3-benzothiazol-2-ylidene)methyl]-1-phenylquinolin-1-ium-2-yl]-N'-propylpropane-1,3-diamine
• InChIKey: CGNLCCVKSWNSDG-UHFFFAOYSA-N
• INCHI: 1S/C32H37N4S/c1-5-20-35(22-13-21-33(2)3)31-23-25(24-32-34(4)29-18-11-12-19-30(29)37-32)27-16-9-10-17-28(27)36(31)26-14-7-6-8-15-26/h6-12,14-19,23-24H,5,13,20-22H2,1-4H3/q+1
• Isómeros SMILES: CCCN(CCCN(C)C)C1=[N+](C2=CC=CC=C2C(=C1)/C=C/3\N(C4=CC=CC=C4S3)C)C5=CC=CC=C5
• Peso molecular: 509.7

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Clase Quinolines and derivatives
      • Subclass Phenylquinolines

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Clase: Quinolines and derivatives
• Subclass: Phenylquinolines
• Intermediate Tree Nodes: Not available
• Direct Parent: Phenylquinolines
• Alternative Parents: Aminoquinolines and derivatives Benzothiazoles Dialkylarylamines Aryl thioethers Aminopyridines and derivatives Pyridinium derivatives Benzene and substituted derivatives Thiazoles Heteroaromatic compounds Trialkylamines Ketene acetals Azacyclic compounds Organopnictogen compounds Hydrocarbon derivatives Organic cations
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Phenylquinoline - Aminoquinoline - Benzo-thiazole - 1,2-benzothiazole - 1,3-benzothiazole - Aryl thioether - Dialkylarylamine - Aminopyridine - Benzenoid - Monocyclic benzene moiety - Pyridinium - Pyridine - Thiazole - Heteroaromatic compound - Tertiary aliphatic amine - Ketene acetal or derivatives - Tertiary amine - Azacycle - Hydrocarbon derivative - Organopnictogen compound - Amine - Organonitrogen compound - Organic nitrogen compound - Organic cation - Aromatic heteropolycyclic compound
• Descripción: This compound belongs to the class of organic compounds known as phenylquinolines. These are heterocyclic compounds containing a quinoline moiety substituted with a phenyl group.
• External Descriptors: cyanine dye - tertiary amine - quinolines - benzothiazolium ion

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Propiedades químicas y físicas

• Sensibilidad: Light sensitive;Moisture sensitive
• Peso molecular: 509.700 g/mol
• XLogP3: 8.200
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 9
• Exact Mass: 509.274 Da
• Monoisotopic Mass: 509.274 Da
• Topological Polar Surface Area: 38.900 Ų
• Heavy Atom Count: 37
• Formal Charge: 1
• Complexity: 734.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 1
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 1
• Covalently-Bonded Unit Count: 1

Referencias

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