Ready-to-use (RTU) for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Product Characteristics
The Synthetic Blocking Buffer, ELISA is a ready-to-use solution for blocking of the remaining free binding sites after microplate coating in ELISAs. The buffer blocks all potential binding sites of nonspecific interaction and reduces the background signal, improving the signal-to-noise ratio.
The Synthetic Blocking Buffer, ELISA reduces the risk of false positive reactions in the assay, while avoiding the current safety hazards related to the use of bovine biological material, for instance BSA.
Composition & Properties
The Synthetic Blocking Buffer is a ready-to-use buffer. The buffer contains no proteins, and crossreactivity is therefore avoided.
Working Procedure
1.Coat the microtiter plate according to the usual procedure. It is recommended to dilute the biomolecule in a fresh carbonate buffer, pH 9.6.
2.After plate coating, rinse the microwells with 3 x 300 µl PBS buffer.
3.Dispense the Synthetic Blocking Buffer into each well making sure wells are completely full.
4.Incubate for 5-10 minutes at room temperature.
5.Rinse with 3 x 300 µl washing buffer optimized for the assay.
6.Continue the plate processing using the usual procedure.
Tips & Tricks
• In case of a demand for stabilization of coated ELISA plates, our one step blocker & stabilizer, WellChampion (cat. no. W494467) is recommended.
• For membranes, the Synthetic Blocking Buffer, Blotting (cat. no. S494457) is recommended.
Handling & Storage
• Store solution at 2-8 °C.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 E2626158 | Certificate of Analysis | Apr 27, 2023 | S494401 |
| F23061791 | Certificate of Analysis | Apr 27, 2023 | S494401 |
| F2306605 | Certificate of Analysis | Apr 27, 2023 | S494401 |
| F2306818 | Certificate of Analysis | Apr 27, 2023 | S494401 |
| F2306867 | Certificate of Analysis | Apr 27, 2023 | S494401 |
| Sensibilidad | Light sensitive |
|---|
