T7 Tag Peptide

Cat. No.: T750879

GRADE & PURITY ≥98%(HPLC), 5mg/ml

Descripción general

The T7 Tag Peptide is commonly used for competitive elution of T7 tag fusion proteins bound to T7 Tag antibodies. It can be used to elute T7 tag fusion proteins from Anti-T7 Magnetic Beads or conventional T7 Tag antibodies during immunoprecipitation.This product competitively elutes the T7 Tag fusion protein, and the eluted sample does not contain the light and heavy chains of T7 Tag antibodies, thus effectively eliminating the interference of antibodies with subsequent Western blot analysis.The main parameters of T7 Tag Peptide are as follows

Precautions：

The solution of this product should be aliquoted and stored at -20℃ or lower temperatures to avoid repeated freeze-thaw cycles.This peptide is easily dissolved in organic solvents such as TFA and slightly soluble in water. For the powder form, it should be prepared with TFA to 100mg/ml first, then diluted to 5mg/ml or lower concentrations with TBS or water. High concentration aqueous solution may precipitate after freeze-thaw.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. For P9819-25mg provided in powder form, centrifuge at 8,000-12,000×g for 10-30 seconds before opening to collect the powder at the bottom of the tube. Dissolve it in TFA by gently pipetting or shaking. Do not vortex vigorously to avoid denaturation and inactivation of the peptide.2. P9819-1mg and P9819-5mg can be directly used for preparing elution buffer. 3. Competitive elution with the T7 Tag Peptide: This elution method is non-denaturing, with high elution efficiency, and the eluted sample does not contain the light and heavy chains of T7 Tag antibodies.a. Preparation of T7 tag elution buffer: Based on the required amount of elution buffer, dilute an appropriate amount of T7 Tag Peptide stock solution with TBS to a final concentration of 150μg/ml.b. Add 100μl of T7 tag elution buffer (150μg/ml) to each immunoprecipitated sample, and incubate on ice or at 4℃ for 30 minutes to 2 hours with shaking. In order to improve the elution efficiency, the incubation time can be extended or the elution can be repeated. The volume of the T7 tag elution buffer is generally 5 times that of the bead or gel suspension.c. Centrifuge at 6000×g for 30 seconds at 4℃, and carefully transfer the supernatant to a new tube. The supernatant contains the T7 tag fusion protein and its protein complexes. Be careful not to touch the resin when taking the supernatnant. 4. Store the eluted T7 tag fusion protein at 4℃ for immediate use, or at -20℃ for long-term storage.

Specifications

Product Name

T7 Tag Peptide

Especificaciones y pureza

≥98%(HPLC), 5mg/ml

Tipo de molécula

Peptide

Almacenamiento y envío

Concentración

5mg/ml

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Stored at 4℃ for up to 1 year.