GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Native?Native grade — protein/biomolecule in its natural (non-recombinant, non-denatured) form. Use when native structure and activity are required.Lyophilized powder?Lyophilized (freeze-dried) powder — gently dried to preserve activity and aid storage. Use for sensitive biologics reconstituted before use.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.from Geobacillus stearothermophilus; ≥7000 PU/mg enzyme powder
BioReactivo, para cultivo celular, polvo,30-350 unidades/mg proteína (E1%/280) Bioactive,Lyophilized powder,Native,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Conservar a -20°C Ships Hielera + almohadillas de hielo Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Metalloendopeptidasa extracelular termoestable (termófila) que contiene cuatro iones de calcio. Los cofactores son el zinc y el calcio. Hidroliza los enlaces proteicos en el lado N-terminal de los residuos de aminoácidos hidrofóbicos. El pH óptimo es 8,0 y la temperatura óptima para la actividad es 70 °C. Considerablemente estable de pH 5 a 9,5. La termolisina tiene una baja especificidad de escisión, por lo que produce una serie de fragmentos cortos que son adecuados para la secuenciación. Escisión preferencial: X-escisión-Y-Z donde X=cualquier aminoácido; Y=Leu, Phe, Ile, Val, Met, Ala y Z es cualquier aminoácido distinto de Pro. El clivaje N-terminal a Leu se prefiere sobre el clivaje N-terminal a Phe que se prefiere sobre los otros. A menudo se utiliza para hacer proteólisis limitada para el mapeo de péptidos y estudios de estructura de proteínas y cambios conformacionales.
Specifications
Product Name
Termolisina de Geobacillus stearothermophilus, CAS No.9073-78-3
from Geobacillus stearothermophilus; ≥7000 PU/mg enzyme powder
Reconstitución
Reconstitute in 42% glycerin (pH 8.0) to a concentration of 1 mg/ml, Dilute it with enzyme diluents (0.01M borate buffer, pH 8.0, containing 0.002M CaSO₄ and 0.005% Triton X-100.).
Condiciones de almacenamiento de almacenamiento
Conservar a -20°C
Enviado en
Hielera + almohadillas de hielo
Estabilidad y almacenamiento
Store at -20℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.
Definición de unidad
One protease unit (PU) is defined as the amount of the enzyme that will liberate non-proteinous digestion products from milk casein (final concentration 0.5%) to give a Folin's color equivalent to 1μg of tyrosine per minute at the early stage of the react
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
1.Xiang Ming, Lin Xiangdong, Chen Haiying. (2025) Luteolin Inhibits Ferroptosis of HUVEC by Regulating the Sirt1/Nrf2 Pathway. CELL BIOCHEMISTRY AND BIOPHYSICS, [PMID:40721691][10.1007/s12013-025-01831-1]
Calculadoras de soluciones
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