Thionin Staining Solution (2%) - BioReagent,Biological Stain,for microscopy,2% , CAS No.78338-22-4

CAS: 78338-22-4 Cat. No.: S1508553 Peso molecular: 287.34 Número EC: 676-854-9 PubChem CID: 2724414

GRADE & PURITY BioReagent, Biological Stain, for Microscopy, 2%

Synonyms

Thionin Blue Staining Solution | Thionine Staining Solution

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining

Descripción general

Many dyes exhibit varying degrees of affinity for DNA and can therefore be used to stain chromosomes. For routine staining of chromosomes, good results are generally obtained with Giemsa, orecin, Feulgen, carbol fuchsin, etc. Thionine can be used for chromosome staining in samples such as oral mucosa, urine, amniotic fluid, chorionic villus cells, and cultured cells, and is particularly suitable for staining sex chromatin, which is often difficult to stain. Additionally, thionine can be used for staining structures such as Nissl bodies and mast cells.

Thionin Staining Solution (2%) is primarily composed of phosphate and thionine. When staining Nissl bodies, they appear dark blue; however, when staining mast cells, they appear purplish‑red. This reagent is intended for research use only and is not suitable for clinical diagnosis or other purposes.

Instructions for Use (For Reference Only)

1. Staining of Nissl Bodies

• Fixation: Use neutral formalin fixative or other appropriate fixatives.

• Paraffin sections 6–8 μm thick; routine deparaffinization with xylene or dewaxing clearing solution to distilled water.

• Immerse the slide specimen in Thionine Staining Solution (2%) for 10–15 minutes; rinse briefly with distilled water.

• Differentiate rapidly with 95% ethanol.

• Dehydrate with absolute ethanol, clear with xylene or dewaxing clearing solution, and mount with neutral balsam.

2. Staining of Chromatin

• Place the slide specimen in 1 mol/L HCl and incubate at 37°C for 20 minutes.

• Rinse thoroughly with distilled water and air‑dry.

• Immerse the slide specimen in Thionine Staining Solution (2%) for 10–15 minutes.

• Rinse with distilled water and air‑dry.

• Under low magnification, search for evenly dispersed cell populations, then carefully observe under oil immersion.

Staining Results

1. Nissl bodies appear dark blue; nuclei appear light blue.

2. Chromatin appears purplish‑red; other components appear blue.

Notes

1. Tissue intended for Nissl body staining should be as fresh as possible and fixed promptly; otherwise, Nissl bodies may dissolve and stain poorly.

2. Nissl body specimens should be stored protected from light, as they are prone to fading.

3. During centrifugation to obtain cell pellets, for certain cell types, if the pellet is insufficient, centrifugation force or time may be increased appropriately.

4. This staining solution is more suitable for Nissl body staining; although it can be used for chromatin staining, it is not recommended.

5. For your safety and health, please wear a lab coat and disposable gloves during operation.

6. Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.

Specifications

Sinónimos

Thionin Blue Staining Solution | Thionine Staining Solution

Especificaciones y pureza

BioReagent, Biological Stain, for microscopy, 2%

Estabilidad y almacenamiento

Store at room temperature long term (12 months). Store in the dark.

Condiciones de almacenamiento de almacenamiento

Protected from light, Room temperature

Enviado en

Normal

Grado

Biological Stain, BioReagent, for Microscopy

Nombres e identificadores

• Isómeros SMILES: CC(=O)[O-].C1=CC2=C(C=C1N)SC3=CC(=[NH2+])C=CC3=N2
• PubChem CID: 2724414
• Peso molecular: 287.34

Propiedades químicas y físicas

• Sensibilidad: Light-sensitive

Referencias

1. Yana Chen, Shuangling Wang, Jujie Ren, Haiyan Zhao, Min Cui, Na Li, Meng Li, Cong Zhang.  (2022)  Electrocatalysis of Copper Sulfide Nanoparticle-Engineered Covalent Organic Frameworks for Ratiometric Electrochemical Detection of Amyloid-β Oligomer.  ANALYTICAL CHEMISTRY,      [PMID:35920591] [10.1021/acs.analchem.2c01602]

2. Huawei Yang, Liangjiu Bai, Donglei Wei, Lixia Yang, Wenxiang Wang, Hou Chen, Yuzhong Niu, Zhongxin Xue.  (2018)  Ionic self-assembly of poly(ionic liquid)-polyoxometalate hybrids for selective adsorption of anionic dyes.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2018.10.100]

3. Xinming Wang, Yiming Zhong, Bei Qian, Shixing Huang, Qiang Long, Haonan Zhang, Qiang Zhao, Xiaofeng Ye.  (2025)  Self-assembled extracellular matrix-lipid nanoparticle composite for site-specific siRNA delivery to improve cardiac repair post-myocardial infarction.  Materials Today Bio,      [PMID:40893381] [10.1016/j.mtbio.2025.102205]