Transferrin Receptor/CD71 Rat mAb (Biotin) - Primary antibody, specific to Transferrin Receptor/CD71

Cat. No.: Ab190201

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

R17 217.1.3

Conjugation

Biotin

Descripción general

Rat anti Mouse Transferrin Receptor/CD71 Antibody, Monoclonal (R17 217.1.3), could be used for Flow, in vivo depletion of CD71+ cells and so on.

Specifications

Product Name

Transferrin Receptor/CD71 Rat mAb (Biotin) - Primary antibody, specific to Transferrin Receptor/CD71

Sinónimos

TFR | Trfr | Mtvr1 | 2610028K12Rik | E430033M20Rik | CD71 antigen | CD71 | CD71TRFR | p90 | T9 | TfR (Transferrin R) | TfR (TransferrinR) | TFR1 | TFRC | TR | transferrin receptor (p90, CD71) | transferrin receptor protein 1 | CD 71 antibody | CD71 antibo

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface wi

Especies hospedadoras

Rat

Especificidad

Transferrin Receptor/CD71

Inmunógeno

Mouse erythroleukemia cell line 745.6

Control positivo

Flow: C57BL/6 mouse bone marrow cells

Conjugación

Biotin

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: Transferrin Receptor/CD71 Rat mAb (Biotin) (Ab190201)

Transferrin Receptor/CD71 Rat mAb (Biotin) (Ab190201) - Flow Cytometry
Flow cytometry analysis of Transferrin Receptor/CD71 expression by C57BL/6 mouse bone marrow cells (right panel) compared to an isotype control (left panel). Cells were stained with Transferrin Receptor/CD71 Rat mAb (Biotin) (Ab190201) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310).

Flow Cytometry: Transferrin Receptor/CD71 Rat mAb (Biotin) (Ab190201)

Transferrin Receptor/CD71 Rat mAb (Biotin) (Ab190201) - Flow Cytometry
Flow cytometry analysis of Transferrin Receptor/CD71 expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were stained with Transferrin Receptor/CD71 Rat mAb (Biotin) (Ab190201) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

Objetivos asociados (humanos)

TFRC Tclin Transferrin receptor protein 1 (0 Activities)

Discover Target: TFRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

TFRC Tclin Transferrin receptor protein 1 (0 Activities)

Discover Target: TFRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de acción