Triticum vulgaris lectin (WGA) , CAS No.96508-08-6

CAS: 96508-08-6 Cat. No.: T140107

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Descripción general

Triticum vulgaris lectin or Weat germ agglutinin (WGA) is isolated from Triticum vulgaris (Weat germ) and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 36 kDa. WGA selectively binds to N-Acetyl glucosamine (GlcNAc) and to N-acetylneuraminic acid (sialic acid) residues of glycoproteins and glycolipids. The WGA lectin agglutinates erythrocytes and most types of malignant cells. It agglutinates rabbit erythrocytes at < 0.1 μg/ml following trypsin treatment of the cells. Adding 300 mM N-Acetyle-D-Glucosamine gives an inhibition with a titer that is at least 8-fold lower than the control. Wheat germ agglutinin inhibits the C5a receptor interaction, which has implications in studies of receptor micro-heterogeneity and ligand binding sites. WGA together with Con A is the lectin most widely used as an analytical and preparative agent when studying glycoproteins and cell surface proteins. The immobilized lectin can be used for affinity chromatography of cells and sub-cellular particles. WGA is supplied as a white to pale yellow lyophilized powder from a buffer containing 10 mM CH3COONH4. No preservatives are added. The purity of WGA is determined by SDS- electrophoresis which generates three bands, corresponding to the three isolectins of WGA. Protein content is verified in spectrophotmetry.
● Ultrapure quality
● Sugar specificity: N-acetylglucosamine and N-acetylneuraminic acid residues
● Agglutinates erythrocytes and most types of malignant cells
● Agglutinates rabbit erythrocytes at ≤0.1 μg/ml after treating the cells with trypsin
● Not blood group specific
Studies of glycoproteins and glycolipids; Purification of membrane proteins; Affinity chromatography of cells and sub-cellular particles; Agglutination studies

Specifications

Mecanismos bioquímicos y fisiológicos

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

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Referencias

1. Qian Wang, Zichao Peng, Bin Gui, Yongming Li, Lanjie Liao, Zuoyan Zhu, Yaping Wang, Libo He.  (2025)  Sialic acid serves as a functional receptor for grass carp reovirus.  PLoS Pathogens,  21  (9): (e1013481).  [PMID:40911632] [10.1371/journal.ppat.1013481]

2. Donghua Su, Xiaolong Zhang, Tianwu An, Xi Wang, Yaqiu Lin, Haibo Wang, Peiqiang Yu, Haitao Shi.  (2025)  Astral-DIA proteomics and 4D label-free N-glycoproteomics of milk fat globule membrane proteins reveal distinct profiles between yak colostrum and mature milk.  Food Chemistry-X,      [PMID:41378274] [10.1016/j.fochx.2025.103302]