UltraBio™ 2× Taq Platinum Universal SYBR Green Premix (ROX Separated) - BioReagent,DNase, RNase free,PCR Reagent,Suitable for molecular biology,for DNA and RNA applications

Cat. No.: FP1509096

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, for DNA and RNA applications

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

PCR, qPCR

Descripción general

  UltraBio™ 2× Taq Platinum Universal SYBR Green Premix (ROX Separated)   is a ready-to-use master mix optimized for quantitative PCR (qPCR) using SYBR Green I dye-based chemistry. It incorporates antibody-mediated hot-start AK Taq V2 Polymerase and a specially formulated buffer, ensuring high specificity, excellent amplification efficiency, and superior detection sensitivity.

  This product includes separate vials of ROX reference dye, making it compatible with a wide range of real-time PCR instruments. Users only need to add primers and template to perform the reaction.

  Intended Use: Quantitative or qualitative detection of nucleic acids using DNA templates. Simply add primers and RNase-free water to complete the reaction.

Product Component Table

FP1509096	Components	100T	1000T	10000T	Storage
FP1509096A	2× Taq Platinum Universal SYBR Green Premix	1 mL	10×1 mL	100mL	-20℃. Store in the dark.
FP1509096B	50× ROX Reference Dye1	40 μL	400 μL	4mL	-20℃.Store in the dark.
FP1509096C	50× ROX Reference Dye2	40 μL	400 μL	4mL	-20℃. Store in the dark.

Key Features

1. High Efficiency & Sensitivity: A novel buffer system combined with a hot-start antibody-modified polymerase ensures high amplification efficiency and sensitivity.

2. Enhanced Specificity: Specially optimized for SYBR Green I chemistry, significantly improving amplification specificity.

3. Simple & Fast: Just add primers and template—no other components are needed.

Protocol

1. Reaction Setup
1.1 Thaw and mix all PCR reagents thoroughly. Briefly centrifuge to bring the solution to the bottom of the tube. It is recommended to aliquot the master mix to avoid repeated freeze-thaw cycles.
1.2 Prepare the PCR reaction according to the table below:

Component	Volume per 20 µL Reaction	Volume per 40 µL Reaction	Final Concentration
2× Taq Platinum Universal SYBR Green Premix	10 μL	20 μL	1×
50× ROX Reference Dye1 (or Dye2)	0.4	0.8	1×
Primer Mix	1 μL	2 μL	/
Template DNA	4 μL	8 μL	/
ddH2O	To 20 μL	To 40 μL	/
Total Volume	20 μL	40 μL

  Note 1: The amount of primers and template can be adjusted according to experimental needs.
  Note 2: 50× ROX Reference Dye 1 is recommended for instruments requiring a high concentration of ROX, such as ABI PRISM® 7000/7300/7900HT, 7900HT Fast, StepOne, and StepOnePlus. 50× ROX Reference Dye 2 is recommended for instruments requiring a low concentration of ROX, such as ABI 7500 and 7500 Fast.

1.3 Mix thoroughly by pipetting gently or vortexing, as the solution is viscous. Briefly centrifuge to collect the liquid at the bottom of the tube.
1.4 Place the reaction tubes in a pre-programmed real-time PCR instrument and start the run.

2. Thermal Cycling Protocol

Note: The reaction conditions can be adjusted based on primer Tm, amplicon length, and instrument requirements.

Specifications

Especificaciones y pureza

BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, for DNA and RNA applications

Estabilidad y almacenamiento

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot. Store in the dark.

Condiciones de almacenamiento de almacenamiento

Protected from light, Store at -20°C, Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology

Propiedades químicas y físicas

• Sensibilidad: Light-sensitive