| Product No. |
Name |
Specification |
Package |
| H752721-RC-10ml |
UltraBio™ His-tag Purification Resin |
Reductant and Chelator-resistant |
10ml |
| H752721-RC-100ml |
UltraBio™ His-tag Purification Resin |
Reductant and Chelator-resistant |
100ml |
| H752721-RC-1L |
UltraBio™ His-tag Purification Resin |
Reductant and Chelator-resistant |
1L |
| H752721-FR-10ml |
UltraBio™ His-tag Purification Resin |
Fast Flow;Resistant to Reductants and Chelators |
10ml |
| H752721-FR-100ml |
UltraBio™ His-tag Purification Resin |
Fast Flow;Resistant to Reductants and Chelators |
100ml |
| H752721-FR-1L |
UltraBio™ His-tag Purification Resin |
Fast Flow;Resistant to Reductants and Chelators |
1L |
| H752721-DR-10ml |
UltraBio™ His-tag Purification Resin |
Denaturant-resistant |
10ml |
| H752721-DR-100ml |
UltraBio™ His-tag Purification Resin |
Denaturant-resistant |
100ml |
| H752721-DR-1L |
UltraBio™ His-tag Purification Resin |
Denaturant-resistant |
1L |
| H752721-FD-10ml |
UltraBio™ His-tag Purification Resin |
Fast Flow;Denaturant-resistant |
10ml |
| H752721-FD-100ml |
UltraBio™ His-tag Purification Resin |
Fast Flow;Denaturant-resistant |
100ml |
| H752721-FD-1L |
UltraBio™ His-tag Purification Resin |
Fast Flow;Denaturant-resistant |
1L | |
Reductant and Chelator-resistant:
Aladdin's UltraBio™ His-tag Purification Resin (Reductant&Chelator-resistant) provides a simple, rapid, and highly efficient method for purifying His-tagged proteins from cell lysates, with high specificity. It is compatible with a certain concentration of reductants and chelants.
A His-tag is a string of histidine residues, typically fused to the N or C terminus of a recombinant protein. The classic his-tag comprises 6 histidine residues, named hexahistidine tag (6xHis-tag). Some recombinant proteins are engineered to have two hexahistidine tags. The His-tag has a high affinity for nickel ions (Ni2+) which is immobilized on
UltraBio™ His-tag Purification Resin. Therefore, the His-tag Purification Resin can be used to purify His-tagged recombinant proteins from a crude lysate, while most other proteins in the lysate will not bind to the resin, or bind only weakly.
UltraBio™
His-tag Purification Resin has a strong affinity for His-tagged proteins, with high specificity and binding capacity, which enables simple, rapid, and efficient purification of the expressed His-tagged protein from a broad host such as bacteria, mammals, insects, Baculovirus, or yeast. The obtained His-tagged protein with high purity can be used for protein structure and function analysis, antibody preparation, protein interaction studies, protein-nucleic acid interaction studies, etc.
UltraBio™
His-tag Purification Resin is based on highly crossed-linked 6% agarose gel on which nickel ions are immobilized by the optimized arm coupling and chelating technology. Compared to other products with similar functions,
UltraBio™ His-tag Purification Resin exhibits lower non-specificity, stronger resistance to pressure, and tighter binding Ni2+ to the resin. This product has minimal leaching of Ni2+ and can be reused many times without reloading nickel ions.
Ni-NTA agarose and most other nickel resin products are not compatible with commonly used concentrations of reducing agents, such as dithiothreitol (DTT) and β -mercaptoethanol, and chelating agents, such as EDTA. However, this product can be compatible with 20mM DTT and 20mM EDTA, facilitating the purification of His-tagged proteins from samples containing reductants and/or chelants. This product is not compatible with 8M urea and 6M guanidine hydrochloride. To purify proteins from inclusion bodies that require 8M urea or 6M guanidine hydrochloride for protein solubilization, dialysis should be performed to remove urea and guanidine hydrochloride before using this product for protein purification.
UltraBio™
His-tag Purification Resin is a ready-to-use Ni2+ chelate blue resin, with a particle diameter of 45-165μm. It can tolerate pressure up to 0.025MPa (~5.8psi). The recommended flow rate is 0.5ml/min when using a fixed flow rate for protein purification.
UltraBio™
His-tag Purification Resin binds His-tagged protein with high capacity and high specificity. Per milliliter gel can bind up to 20-30mg of His-tagged protein. The maximum binding capacity in actual use depends on the molecular weight and characteristics of the protein to be purified. The larger the molecular weight, the greater the maximum binding capacity, and vice versa. For proteins with the same molecular weight, the maximum binding capacity of this resin might be different due to different characteristics of the proteins.
This product is stored in 30% ethanol. A total volume of 10ml contains 5ml of gel and 5ml of liquid. It is advisable to fully resuspend the gel prior to use.
Per 10ml of this product can purify up to 100-150mg of His-tagged recombinant protein.
Precautions：
Store the resin at 4ºC upon receipt. Do NOT freeze.
During the use of this product, the concentration of buffer reagents such as Tris, HEPES, and MOPS, should not exceed 100mM, EDTA should not exceed 20mM, SDS and sarkosyl should not exceed 0.3%, and the concentration of Triton, Tween, and NP-40 should not exceed 2%. The concentration of sodium deoxycholate and CHAPS should not exceed 1%, histidine should not exceed 20mM, calcium ions should not exceed 5mM, sodium and magnesium ions can be up to 2M, and glycerol can be up to 50%. The compatibility of other reagents not mentioned is yet to be tested.
Keep the gel moist during storage or purification.
A 0.45μm filter membrane can be used to remove particulates from cell lysates that could not be completely removed by centrifuge.
Protein samples should be purified after collection as soon as possible and kept at 4ºC or on ice to minimize protein degradation or denaturation. Protein degradation can also be inhibited by the addition of Protease Inhibitor Cocktail.
If protein purification cannot be achieved ideally when using the conditions provided in this manual, optimize imidazole concentration and/or the pH of wash and elution buffers.
This product is for R&D only. Not for drug, household, or other uses.
For your safety and health, please wear a lab coat and disposable gloves during the operation.
Fast Flow;Resistant to Reductants and Chelators:
Aladdin's UltraBio™ His-tag Purification Resin (Fast Flow, Reductant&Chelator-resistant) provides a simple, rapid, highly efficient and specific method for purifying His-tagged proteins from cell lysates, with fast flow rate. This new developed resin is high-pressure tolerant and is compatible with reducing and chelating agents.
A His-tag is a string of histidine residues, typically fused to the N or C terminus of a recombinant protein. The classic his-tag comprises 6 histidine residues, named hexahistidine tag (6xHis-tag). Some recombinant proteins are engineered to have two hexahistidine tags. The His-tag has a high affinity for nickel ions (Ni2+) which is immobilized on UltraBio™ His-tag Purification Resin. Therefore, the UltraBio™ His-tag Purification Resin can be used to purify His-tagged recombinant proteins from a crude lysate, while most other proteins in the lysate will not bind to the resin, or bind only weakly.
This product has high affinity for His-tagged proteins, with high specificity and binding capacity, which enables simple, rapid, and efficient purification of the expressed His-tagged protein from a broad host such as bacteria, mammals, insects, Baculovirus, or yeast. The obtained His-tagged protein with high purity can be used for protein structure and function analysis, antibody preparation, protein interaction studies, protein-nucleic acid interaction studies, etc.
his product employs the highly cross-linked 6% agarose gel on which nickel ions are immobilized by the optimized arm coupling and chelating technology. Compared to other products with similar functions, UltraBio™ His-tag Purification Resin (Fast Flow, Reductant&Chelator-resistant) exhibits lower non-specificity, stronger resistance to pressure, and tighter binding Ni2+ to the resin. This product almost has no leaching of Ni2+ and can be reused many times without the need of reloading nickel ions.
Ni-NTA agarose and most similar nickel column products cannot tolerate common concentrations of reducing agents such as dithiothreitol (DTT), β-mercaptoethanol, etc., as well as common concentrations of chelating agents such as EDTA, etc., in protein samples; however, this product can well tolerate reducing agents such as DTT and chelating agents such as EDTA. This product can tolerate 0.01 M HCl, 0.01 M NaOH (test condition: 40°C, 7d); 10 mM EDTA, 5 mM DTT, 5 mM TCEP, 20 mM β-Mercaptoethanol, 1 M NaOH, 4 M Guanidine hydrochloride (test condition: 25°C, 24h) ; 500 mM imidazole, 100 mM EDTA (test conditions: 25℃, 2 h); 30% Isopropanol (test conditions: 25℃, 20 min). This offers the possibility of purifying protein samples that require the addition of reducing or chelating agents.
This product is a ready-to-use Ni2+ chelate blue resin, with a particle diameter of 45-165μm. It can tolerate pressure up to 0.3MPa (~43.5 psi). The recommended flow rate is 600cm/h when using a fixed flow rate for protein purification.
UltraBio™ His-tag Purification Resin binds His-tagged protein with high capacity and high specificity. Per milliliter gel can bind up to 15 mg of His-tagged protein. The maximum binding capacity in actual use depends on the molecular weight and characteristics of the protein to be purified. The larger the molecular weight, the greater the maximum binding capacity, and vice versa. For proteins with the same molecular weight, the maximum binding capacity of this resin might be different due to different characteristics of the proteins.
This product is stored in 20% ethanol. A total volume of 10ml contains 5ml of gel and 5ml of liquid.
Precautions:
Store at 2-8 ℃. Do NOT freeze.
This product is for RUO only. Not for drug, household, or other uses.
For your safety and health, please wear a lab coat and disposable gloves during the operation.
Denaturant- resistant:
Aladdin's UltraBio™
His-tag Purification Resin (Denaturant-resistant) provides a simple, rapid, and highly efficient method for purifying His-tagged proteins from cell lysates under either native or denaturing conditions, with high specificity. This product is compatible with strong denaturants, such as urea and guanidine hydrochloride.
A His-tag is a string of histidine residues, typically fused to the N or C terminus of a recombinant protein. The classic His-tag comprises 6 histidine residues, named hexahistidine tag (6xHis-tag). Some recombinant proteins are engineered to have two hexahistidine tags. His-tag has a high affinity for nickel ions (Ni2+) which is immobilized on
UltraBio™
His-tag Purification Resin. Therefore, the
UltraBio™
His-tag Purification Resin can be used to purify His-tagged recombinant proteins from a crude lysate, while most other proteins in the lysate will not bind to the resin, or bind only weakly.
UltraBio™
His-tag Purification Resin has a strong affinity for His-tagged proteins, with high specificity and binding capacity, which enables simple, rapid, and efficient purification of His-tagged protein from a broad host such as bacteria, mammals, insects, Baculovirus, or yeast. The obtained His-tagged protein with high purity can be used for protein structure and function analysis, antibody preparation, protein interaction studies, protein-nucleic acid interaction studies, etc.
UltraBio™ His-tag Purification Resin is based on highly crossed-linked 6% agarose gel on which nickel ions are immobilized by the optimized arm coupling and chelating technology. Compared to other products with similar functions,
UltraBio™
His-tag Purification Resin exhibits much lower non-specificity.
UltraBio™
His-tag Purification Resin (Denaturant-resistant) is compatible with 8M urea and 6M guanidine hydrochloride, but not reductants and chelators. Reductants, such as dithiothreitol (DTT) and β -mercaptoethanol, and chelating agents, such as EDTA and EGTA, will cause the leakage of Ni2+.
UltraBio™
His-tag Purification Resin is a ready-to-use Ni2+ chelate blue resin, with a particle diameter of 45-165μm. It can tolerate pressure up to 0.025MPa (~5.8psi). The recommended flow rate is 0.5ml/min when using a fixed flow rate for protein purification.
UltraBio™
His-tag Purification Resin binds His-tagged protein with high capacity and high specificity. Per milliliter of gel can bind up to 25-35mg of His-tagged protein. The maximum binding capacity in actual use depends on the molecular weight and characteristics of the protein to be purified. The larger the molecular weight, the greater the maximum binding capacity, and vice versa. For proteins with the same molecular weight, the maximum binding capacity of this resin might be different due to different characteristics of the proteins.
This product is stored in 20% ethanol. A total volume of 10ml contains 5ml of gel and 5ml of liquid. It is advisable to fully resuspend the gel prior to use.
10ml of this product can purify up to 100-200mg of His-tagged recombinant protein with a molecular weight of 55kD.
Precautions：
Store the resin at 4℃ upon receipt. Do NOT freeze.
During the use of this product, the concentration of buffer reagents such as Tris, HEPES, and MOPS, should not exceed 100mM. The concentrations of Triton X-100, Tween-20, and NP-40 should not exceed 2%. The concentration of sodium deoxycholate and CHAPS should not exceed 1%, histidine should not exceed 20mM, calcium ions should not exceed 5mM, sodium and magnesium ions can be up to 2M, and glycerol can be up to 50%. This product is compatible with urea up to 8M, and guanidine hydrochloride up to 6M, but not compatible with reductants, chelating agents, or strongly acidic or alkaline solutions. The compatibility of other reagents not mentioned is yet to be tested.
Keep the gel moist during storage or purification.
A 0.45μm filter membrane can be used to remove particulates from cell lysates that could not be completely removed by centrifuge.
Protein samples should be purified after collection as soon as possible and kept at 4℃ or on ice to minimize protein degradation or denaturation. Protein degradation can also be inhibited by the addition of Protease Inhibitor Cocktail.
If protein purification cannot be achieved ideally when using the conditions provided in this manual, optimize imidazole concentration and/or the pH of wash and elution buffers.
This product is for R&D only. Not for drug, household, or other uses.
For your safety and health, please wear a lab coat and disposable gloves during the operation.
Fast Flow;Denaturant-resistant:
Aladdin's
UltraBio™
His-tag Purification Resin (Fast Flow, Denaturant-resistant) provides a simple, rapid, highly efficient and specific method for purifying His-tagged proteins from cell lysates, with fast flow rate. This new developed resin is high-pressure tolerant and is compatible with strong protein denaturants such as urea and guanidine hydrochloride.
A His-tag is a string of histidine residues, typically fused to the N or C terminus of a recombinant protein. The classic his-tag comprises 6 histidine residues, named hexahistidine tag (6xHis-tag). Some recombinant proteins are engineered to have two hexahistidine tags. The His-tag has a high affinity for nickel ions (Ni2+) which is immobilized on
UltraBio™
His-tag Purification Resin. Therefore, the
UltraBio™
His-tag Purification Resin can be used to purify His-tagged recombinant proteins from a crude lysate, while most other proteins in the lysate will not bind to the resin, or bind only weakly.
Store at 4℃ for up to 1 year.
This product has high affinity for His-tagged proteins, with high specificity and binding capacity, which enables simple, rapid, and efficient purification of the expressed His-tagged protein from a broad host such as bacteria, mammals, insects, Baculovirus, or yeast. The obtained His-tagged protein with high purity can be used for protein structure and function analysis, antibody preparation, protein interaction studies, protein-nucleic acid interaction studies, etc.
This product employs the highly cross-linked 6% agarose gel on which nickel ions are immobilized by the optimized arm coupling and chelating technology. Compared to other products with similar functions,
UltraBio™ His-tag Purification Resin (Fast Flow, Reductant&Chelator-resistant) exhibits lower non-specificity, stronger resistance to pressure, and tighter binding Ni2+ to the resin.
This product can tolerate 8M urea or 6M guanidine hydrochloride and can be used for the purification of His-tagged proteins under native or denaturing conditions. Reducing agents such as DTT and β-mercaptoethanol will reduce chelated nickel ions, while strong chelating agents such as EDTA/EGTA and other divalent ions will de-nickel the product. Therefore, reducing and chelating agents should not be contained in the solution used for protein purification. For protein purification in the presence of strong reducing agents such as DTT/β-mercaptoethanol or chelating agents such as EDTA/EGTA.
This product is a ready-to-use Ni2+ chelate blue resin, with a particle diameter of 45-165μm. It can tolerate pressure up to 0.3MPa (~43.5 psi). The recommended flow rate is 0.5ml/min when using a fixed flow rate for protein purification.
This product binds His-tagged proteins with high capacity and specificity. The maximum protein binding capacity is 25-35mg protein (with a molecular weight of 55kD)/ml gel. The maximum binding capacity in practice depends on the molecular weight and characteristics of the His-tagged recombinant protein to be purified. The larger the molecular weight, the higher the maximum binding capacity, and vice versa. However, for proteins with the same molecular weight, the maximum binding capacity may vary depending on the properties of the purified protein.
This product is stored in 20% ethanol. A total volume of 10ml of this product contains 5ml of gel and 5ml of liquid. It is advisable to fully resuspend the gel prior to use.
Up to 100-200mg of His-tagged recombinant protein (with a molecular weight of 55kD) can be purified per 10ml of this product.
Precautions：
Store the resin at 4℃ upon receipt. Do NOT freeze.
During the use of this product, the concentration of buffer reagents such as Tris, HEPES, and MOPS, should not exceed 100mM. The concentrations of Triton X-100, Tween-20, and NP-40 should not exceed 2%. The concentration of sodium deoxycholate and CHAPS should not exceed 1%, histidine should not exceed 20mM, calcium ions should not exceed 5mM, sodium and magnesium ions can be up to 2M, and glycerol can be up to 50%. This product is compatible with urea up to 8M and guanidine hydrochloride up to 6M, but not compatible with reductants, chelating agents, or strongly acidic or alkaline solutions. The compatibility with other reagents not mentioned is yet to be tested.
Keep the gel moist during storage or purification.
A 0.45μm filter membrane can be used to remove particulates from cell lysates that could not be completely removed by centrifuge.
Protein samples should be purified after collection as soon as possible and kept at 4℃ or on ice to minimize protein degradation or denaturation. Protein degradation can also be inhibited by the addition of Protease Inhibitor Cocktail.
If protein purification cannot be achieved ideally when using the conditions provided in this manual, optimize imidazole concentration and/or the pH of wash and elution buffers.
This product is for R&D only. Not for drug, household, or other uses.
For your safety and health, please wear a lab coat and disposable gloves during the operation.
