UltraBio™ Small DNA Clean Beads - BioReagent, DNase, RNase free, Suitable for molecular biology, for DNA and RNA applications

Cat. No.: S1456145

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, for DNA and RNA applications

Synonyms

Smarter DNA Clean beads

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Application

Nucleic Acid Extraction and Purification

Descripción general

UltraBio™ Small DNA Clean Beads designed for efficient purification of DNA fragments ≥50 bp. Enables rapid removal of primer dimers, dNTPs, salts, and proteins. Purified DNA is directly suitable for sequencing, PCR templates, enzymatic digestion, and other downstream applications.

User-Supplied Reagents & Equipment

1.Reagents

80% Ethanol

Elution Buffer: 10 mM Tris-HCl (pH 8.0) or Nuclease-Free Water

2.Equipment

Pipettes

Vortex mixer

Magnetic rack

1.5 mL microcentrifuge tubes

Manual Protocol (Centrifuge Tubes)

                       Table 1: Bead-to-Sample Ratios

DNA Fragment Size	≥50 bp	≥200 bp	≥500 bp
Bead Suspension Volume	2.2×	1.0×	0.5×

1.Binding

Add bead suspension (volume per Table 1) to sample in 1.5 mL tube.

Vortex-mix → Incubate 5 min at RT.

Place tube on magnetic rack → Discard supernatant when clear.

2.Wash 1

Keep tube on rack. Add 200 μL 80% ethanol (submerge beads).

Incubate 1 min → Discard supernatant (do not resuspend).

3.Wash 2

Repeat Step 2.

4.Ethanol Removal

Air-dry beads 2–5 min (critical: avoid over-drying).

5.Elution

Add 30–100 μL Elution Buffer.

Vortex-mix → Incubate 5 min at RT.

6.DNA Recovery

Place tube on magnetic rack 2–5 min.

Transfer supernatant to new tube.

Automated Protocol: 16/32-Channel System

                                                                       96-Well Plate Setup

Well Position	1、7	2、8	3、9	6、12
Reagent	Sample + Beads (vol. per Table 1)	200 μL 80% ethanol	200 μL 80% ethanol	30–100 μL Elution Buffer

Procedure

1.Load plate into instrument → Insert magnetic tips.

2.Execute predefined program.

3.Transfer eluate from columns 6 & 12 to nuclease-free tubes.

Automated Protocol: 96-Channel System

                                       96-Well Plate Setup

Plate Position	1	2	3	6
Reagent per Well	Sample + Beads (vol. per Table 1)	200 μL 80% ethanol	200 μL 80% ethanol	40–100 μL Elution Buffer

Procedure

1.Load plate → Insert magnetic tips.

2.Run instrument program.

3.Seal elution plate or transfer to nuclease-free PCR plate.

Storage: -20°C

Precautions

1.Bead Handling

DO NOT freeze or centrifuge the suspension.

Vortex thoroughly before first use.

2.Sample Volume

Minimum 100 μL (adjust to 100 μL with water if needed).

3.Pre-Use Protocol

Equilibrate beads to RT for ≥30 min.

4.Critical Step

Avoid over-drying beads during ethanol removal.

Specifications

Sinónimos

Smarter DNA Clean beads

Especificaciones y pureza

BioReagent, DNase, RNase free, Suitable for molecular biology, for DNA and RNA applications

Estabilidad y almacenamiento

Store at 2-8℃ long term (12 months). Do not freeze.

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C, Do not freeze

Enviado en

Wet ice, Do not freeze

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent, DNase, RNase free, for DNA and RNA applications, Suitable for molecular biology