UltraBio™ StableMax RT-qPCR Master Mix (Dye, UDG, One Tube) - BioReagent,DNase, RNase free,PCR Reagent,Suitable for molecular biology,for DNA and RNA applications,10 μL/T

Cat. No.: FP1508907

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, for DNA and RNA applications, 10 μL/T

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

PCR, RT-qPCR

Descripción general

  The StableMax RT-qPCR Master Mix (with Dye, UDG) is a one-tube, probe-based RT-qPCR premix optimized for both single and multiplex RNA detection. Its unique buffer system is compatible with Taq DNA polymerase and reverse transcriptase, enabling seamless reverse transcription and amplification in a single reaction.

Catalog	Component	100T	1000T	10000T	Storage
U1492577	UltraBio™ StableMax RT-qPCR Master Mix (Dye, UDG, One Tube)	1.0 mL	10×1.0 mL	100mL	-20℃

Key Features:
✔ Visual tracking: Yellow dye (non-inhibitory) confirms reagent addition, preventing omissions
✔ Contamination control: dUTP/UDG system eliminates carryover amplicon contamination
✔ Broad applications: Suitable for one-step RT-qPCR (RNA targets) and hot-start DNA amplification
✔ Optimized performance: High sensitivity and reproducibility for low-abundance targets

Protocol

I. Reaction Setup

1. Reagent Preparation

   • Thaw all components at room temperature or 4°C.

   • Mix gently and centrifuge briefly before use.

   • Recommendations:

     • Protect probes from light.

     • Aliquot master mix to avoid freeze-thaw cycles.

2. Prepare Reaction Mix

Component	Volume per 25 μL	Volume per 50 μL	Final Concentration
StableMax RT-PCR Master Mix (Dye, UDG, One Tube)	10 μL	20 μL	1×
Primer-probe Mix	1 μL	2μL	/
Templates	5 μL	10 μL	/
ddH₂O	Up to 25 μL	Up to 50 μL	/
Total	25 μL	50 μL	/

*Note:

• Primer/template volumes can be adjusted.

• For multiplex assays, optimize primer concentrations empirically.

1. Mixing:

   • Gently pipette mix or vortex briefly.

   • Centrifuge at room temperature to collect liquid.

2. PCR Amplification:

   • Load tubes onto a thermal cycler with heated lid (105°C recommended).

II. Thermal Cycling Program

Step	Temperature	Time	Cycles
1	50℃	15min	1
2	95℃	2min 30s	1
3	94℃	10s	45
4	55℃	40s（Fluorescence read）	45

Optimization Guidelines:
a. Reverse Transcription: 5–15 min (adjust based on target abundance).
b. Cycle Number: Reduce to 40 cycles for high-copy targets.
c. Primer Design:

• Final concentration: 0.2 μM (typical).

• Tm ≥55°C (use software for accurate calculation).

• Adjust annealing temperature (±3°C) if amplification is suboptimal.

Specifications

Especificaciones y pureza

BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, for DNA and RNA applications, 10 μL/T

Estabilidad y almacenamiento

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot.

Condiciones de almacenamiento de almacenamiento

Store at -20°C, Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology