UltraBio™ StableMax RT-qPCR Master Mix (UDG, One Tube) - BioReagent,DNase, RNase free,PCR Reagent,Suitable for molecular biology,for DNA and RNA applications,10 μL/T

Cat. No.: FP1508908

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, for DNA and RNA applications, 10 μL/T

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

PCR, RT-qPCR

Descripción general

  StableMax RT-qPCR Master Mix (with UDG) is a ready-to-use, all-in-one probe-based master mix for single/multiplex RT-qPCR detection of RNA targets. Optimized buffer simultaneously supports Taq DNA polymerase and reverse transcriptase activity, ensuring broad target compatibility. Pre-mixed working solutions containing primers/probes remain stable at low temperatures without compromising performance. Incorporates dUTP/UDG contamination control to eliminate carryover amplicon contamination. Compatible with standard and fast cycling protocols.

Component

Catalog	Component	100T	1000T	10000T	Storage
FP1508908	UltraBio™ StableMax RT-qPCR Master Mix (UDG, One Tube)	1.0 mL	10×1.0 mL	100mL	-20℃

  Applications:

• One-step RT-qPCR (RNA targets)

• Hot-start DNA amplification

Key Advantages

1. Unified Buffer System: Optimized for both Taq polymerase and reverse transcriptase.

2. Ready-to-Detect: Add primers/probes to master mix → store as working solution → add sample for direct use.

3. Contamination Control: dUTP/UDG system cleaves uracil-containing contaminants.

4. Broad Compatibility: Adapts to fast cycling (≤40 min) and multiplex assays.

Protocol

1. Reaction Setup

Component	Volume/25 μL	Volume/50 μL	Final Conc.
StableMax RT-qPCR Master Mix	10 μL	20 μL	1×
Primer-probe Mix	1 μL	2μL	/
Templates	5 μL	10 μL	/
ddH₂O	Up to 25 μL	Up to 50 μL	/
Total	25 μL	50 μL	/

Notes:

• Primer/template volumes are adjustable.

• For fast cycling, use 25 μL total volume.

• Protect probes from light; avoid freeze-thaw cycles.

2. Mixing & Centrifugation

• Gently pipette-mix or vortex lightly.

• Centrifuge briefly to collect liquid.

3. Cycling Conditions

Step	Temperature	时间	循环数
1	50℃	15min	1
2	95℃	2min 30s	1
3	94℃	10s	45
4	55℃	40s（collect fluorescence）	45

Notes

1. Reverse transcription (adjust: 5–15 min)
2. Initial denaturation (adjust: 1.5–2.5 min)
3. Reduce to 40 cycles for high-copy targets.
4. Primer Design:
Final concentration: 0.2 μM
Tm ≥55°C (design with tools like OligoAnalyzer™).

Specifications

Especificaciones y pureza

BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, for DNA and RNA applications, 10 μL/T

Estabilidad y almacenamiento

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot.

Condiciones de almacenamiento de almacenamiento

Store at -20°C, Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology