UltraBio™ Streptavidin Magnetic Beads

Cat. No.: S751585

Descripción general

Aladdin's UltraBio™ Streptavidin Magnetic Beads are superparamagnetic beads, with 200nm particle size, covalently coupled with high-quality streptavidin that binds with biotin ligands specifically, rapidly, and highly efficient. The beads can be used for immunoprecipitation (IP), protein interaction studies, DNA-protein pulldowns, and purification of biotin-labeled proteins and nucleic acids.

Streptavidin coupled to UltraBio™ Magnetic Beads has a mass of 55kD and 4 biotin-binding sites. It binds to biotin with extremely high affinity (Kd=10-15M). Streptavidin is a functional and structural analog to avidin. However, unlike avidin, streptavidin is non-charged due to the absence of carbohydrate groups and its near-neutral isoelectric point (PI), resulting in low nonspecific binding under neutral conditions. Therefore streptavidin instead of avidin is more regularly used in biotinylation-relevant studies.

UltraBio™ Streptavidin Magnetic Beads bind with biotin-labeled proteins and nucleic acids, and have wide applications in biomedical studies, such as acting as a carrier for IP, cell sorting and ELISA, isolating DNA/RNA-protein complex, DNA/RNA hybridization, isolating mRNA, ssDNA oligonucleotides and PCR products, etc.

Product Advantages
Compared to most other similar products, the UltraBio™ Streptavidin Magnetic Beads bind with biotin-labeled molecules in complex biological samples with higher capacity and lower nonspecificity due to its more condensed streptavidin coupling and smaller particular size. Per milliliter of the beads suspension contains 10mg of magnetic beads.

UltraBio™ Streptavidin Magnetic Beads bind with biotin-labeled molecules rapidly and high efficiently. The nanometer magnetic bead (~200nm) has a large specific surface area, facilitating a fast and efficient binding between streptavidin and biotin-labeled molecules. A shorter operation time can effectively minimize the degradation or denaturation of biotin-labeled molecules.

Because of the low nonspecific binding of UltraBio™ Streptavidin Magnetic Beads, the highly purified biotin-labeled molecules are compatible with mass spectrometry analysis.

Precautions
1. Before using magnetic beads, they should be thoroughly mixed to prevent changes in the concentration of the beads and to avoid prolonged ultrasonic damage to the surface of the beads;

2. Before using magnetic beads, please perform magnetic separation and clean 2-3 times with pure water or the buffer solution used;

3. During use and storage, avoid freezing and thawing.

Specifications

Product Name

UltraBio™ Streptavidin Magnetic Beads

Conjugación

Magnetic beads

Tipo de molécula

Bioreagents/Buffer

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Estabilidad y almacenamiento

Store at 4℃ for up to 2 years, or at -20℃ for long-term storage.