UltraBio™ Thanos Poly Active PCR Mix

Cat. No.: FP1508890

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, for DNA and RNA applications, 16μL/T

Descripción general

 Thanos Poly Active PCR Mix is a fluorescent quantitative premix containing hot-start antibody enzymes. It not only supports full premixing with primers and probes but also exhibits strong adaptability for multiplex DNA assays. To reduce tedious experimental operations at the end-user stage, the primer-probe premix can be prepared in advance, and samples can be directly added during detection.This product uses hot-start antibody enzymes combined with an exclusively developed buffer for the full premix system. It greatly enhances the stability of the amplification system and the detection sensitivity of DNA samples. It also shows strong adaptability and versatility in qPCR assays such as respiratory tract, diarrhea, HPV, and veterinary pathogen detection.

Catalog	Component	100T	1000T	10000T	Storage
FP1508890	UltraBio™ Thanos Poly Active PCR Mix	1.6 mL	10×1.6 mL	160mL	-20℃

Application Scope

Quantitative or qualitative nucleic acid detection using DNA as the template. Only primers, probes, and supplementary ddH2O need to be added.

Product Advantages

1. Novel combination of independently developed buffer and hot-start antibody enzymes ensures high specificity and sensitivity of reactions.
2. Ready-to-use working premix can be prepared by simply adding primers and probes; samples are added directly during detection.
3. Integrated dUTP/UDG anti-contamination system effectively prevents aerosol contamination.
4. Compatible with rapid amplification and multiplex target amplification.

Usage Instructions

Reaction System Preparation

1. Dissolve and mix all solutions required for the PCR reaction thoroughly. After brief centrifugation, place at room temperature for later use. Probes should be protected from light. It is recommended to aliquot PCR solutions for use to avoid repeated freeze-thaw cycles.

2. Prepare the PCR reaction system according to the following table:

Component	Volume per 25 µL Reaction	Volume per 50 µL Reaction	Final Concentration
Thanos Poly Active PCR Mix	16 μL	32 μL	1×
Primer/Probe Mix	1 μL	2 μL	/
Template DNA	5 μL	10 μL	/
ddH₂O	To 25 μL	To 50 μL	/
Total Volume	25 μL	50 μL

Note 1: The amounts of primers and template can be adjusted as needed.

Note 2: When using a rapid reaction program, it is recommended to set the total volume of the reaction system to 25μL.

3. Gently mix by pipetting up and down or slight vortexing. Centrifuge at room temperature for a few seconds to collect the liquid at the bottom of the tube.

4. Place each PCR reaction tube on the pre-set PCR instrument and start the PCR reaction.

Step	Temperature	Time	Cycles
Pre-denaturation	95℃	1min 30s	1
Denaturation	95℃	5s	40
Annealing/Extension	55℃	30s (Acquire fluorescence)	40

Note: The reaction conditions can be adjusted according to actual circumstances.

Specifications

Product Name

UltraBio™ Thanos Poly Active PCR Mix

Grado

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology

Especificaciones y pureza

BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, for DNA and RNA applications, 16μL/T

Tipo de molécula

Bioreagents/Buffer

Almacenamiento y envío

Concentración

16μL/T

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot. Store in the dark.