V5 Tag Peptide

Cat. No.: V751701

GRADE & PURITY ≥98%(HPLC), 5mg/ml

Descripción general

V5 Tag Peptide is a commonly used to elute V5-tagged fusion proteins from Anti-V5 Tag antibodies coupled with magnetic beads or other resins via competitive bindings during immunoprecipitation.This product elutes the V5-tagged proteins specifically and the eluted sample does not contain Anti-V5 Tag antibodies, which effectively eliminates their interference with the subsequent analysis such as Western Blot.V5 Tag Peptide is a synthetic peptide with 14 amino acids (GKPIPNPLLGLDST).The main characteristics of V5 Tag Peptide are listed below

Precautions：

The stock solution of this product should be aliquoted and stored at -20℃ or lower temperature to avoid repeated freeze-thaws.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.only trace amounts of peptides are deposited in the tube during the lyophilization. Before opening the tube, we recommend centrifuging the tube at approximately 8,000-12,000×g for 10-30 seconds to collect the peptides to the bottom of the tube.2.For P9813-25mg, dissolve the peptide in TBS completely by pipetting or flicking the tube gently. Do not vortex to avoid the denaturation of the peptide.3.Competitive elution of V5-tagged proteins from immunoprecipitated samples: This is a non-denaturing method with high elution efficiency and the eluted sample does not contain the Anti-V5 Tag antibody.a.Preparation of the V5 Tag elution buffer: Based on the experimental needs, prepare an appropriate amount of elution buffer by diluting the V5 Tag Peptide stock solution to 150μg/ml with TBS.b.Add 100μl of V5 Tag elution buffer (150μg/ml) to each immunoprecipitated sample and incubate on ice or at 4℃ with shaking for 0.5-2h. To increase the elution efficiency, the incubation time can be extended or the elution can be repeated. The volume of V5 Tag elution buffer is generally 5 times of the initial volume of the gel suspension or magnetic bead suspension.c.Centrifuge at 6,000×g for 30 seconds at 4℃ and carefully transfer the supernatant to a new tube. The supernatant contains the V5-tagged proteins or their protein complexes.4.Store the eluted V5-tagged proteins or protein complexes at 4℃ for immediate use, or -20℃ for long term storage.

Specifications

Product Name

V5 Tag Peptide

Especificaciones y pureza

≥98%(HPLC), 5mg/ml

Tipo de molécula

Peptide

Almacenamiento y envío

Concentración

5mg/ml

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Stored at 4℃ for up to one year.