GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Suitable for electrophoresis?Electrophoresis grade — low ionic/UV impurities for clean gel runs. Use in agarose/PAGE electrophoresis where impurities distort bands.Ready-to-use?Ready-to-use — supplied pre-formulated at working concentration, no prep needed. Use to save time and reduce pipetting/dilution errors.for Western blot?Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low.
BioReagent, ready-to-use, suitable for electrophoresis, for western blot BioReagent,for Western blot,Ready-to-use,Suitable for electrophoresis for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Eliminates the need to waste precious samples on repeated gel electrophoresis for detecting different primary antibodies. Simply treat the membrane with this stripping buffer to remove the first primary antibody. This process typically takes 10–20 minutes, depending on the amount of antibody bound to the membrane. After stripping, the membrane can be reprobed with a new primary antibody.
Key Features
Saves samples: The same membrane with identical samples can be used for multiple detections.
Time-efficient: No need to rerun gel electrophoresis.
Highly effective: Superior stripping performance compared to homemade buffers.
Gentle formulation: Minimal damage to target proteins on the membrane.
Odorless: No pungent odor, more environmentally friendly.
Protocol
After completing Western Blot chemiluminescence detection, rinse the membrane with TBS/T or PBS/T for 5 minutes.
Add 10–20 mL of stripping buffer to cover the membrane. Incubate on a shaker for 10–20 minutes (duration depends on the amount of antibody bound).
Discard the stripping buffer and rinse the membrane with TBS/T or PBS/T for 5 minutes.
Optional: Apply chemiluminescence substrate to quickly verify stripping efficiency.
Reblock the membrane with an appropriate blocking buffer before reprobing with other antibodies.
The membrane can be stripped at least 10 times without significantly affecting signal intensity.
Example: GAPDH signals showed no significant reduction after 6 hours of continuous stripping (equivalent to 24 cycles at 15 min each).
Specifications
Especificaciones y pureza
BioReagent, ready-to-use, suitable for electrophoresis, for western blot
Estabilidad y almacenamiento
Store at 2-8℃ long term (12 months).
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C
Enviado en
Wet ice
Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.
Grado
BioReagent, for Western blot, Ready-to-use, Suitable for electrophoresis
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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