Western Rapid Transfer Buffer (10X, Wet & Semi-dry) - BioReagent, for western blot, sterile-filtered, 10X

Cat. No.: W743360

GRADE & PURITY Sterile-filtered, BioReagent, for Western blot, 10X

Synonyms

Transfer Buffer (10X)

Storage

Store at 2-8°C

Shipped In

Wet ice

Descripción general

Aladdin's Western rapid membrane transfer solution (10 ×) is an efficient membrane transfer solution independently developed by Aladdin Biotech, which can be used for wet or semi dry membrane transfer. It can efficiently and quickly transfer proteins to PVDF or nitrocellulose membranes, and the heat generated during the transfer process is much smaller than that of ordinary membrane transfer buffer, thereby reducing protein loss.
This product is safe and non-toxic. Before use, it only needs to be diluted with water and ethanol to 1 x (which can quickly complete the entire film transfer process within 15-35 minutes). This product has high film transfer efficiency and can quickly complete the film transfer process within 15-35 minutes. This product has high stability, low heat generation, and reduces protein loss. This product has good compatibility and is also applicable to proteins with large molecular weight span. It can effectively solve the problem that large and small proteins cannot be transferred on the same membrane at the same time. It is compatible with Tris Gly system, Hepes system, Bis Tris system and other gel.
Precautions：
1. When the transfer solution is stored at 2-8 ℃ for a long time, if precipitation occurs, it can be incubated in a water bath and fully dissolved before continuing to be used.
2. When using ethanol, it is not necessary to use anhydrous ethanol. If using aqueous ethanol, please calculate the amount added according to the actual concentration of ethanol in the reagent.
3. When wet rotating the film, there is no need for an additional ice bath outside the electrospinning device to ensure no overheating phenomenon. If you want to complete the film rotation more quickly, you can use a constant current of 400mA or higher to circulate the film.
4. This product is for scientific research purposes only and should not be used for any other purposes.
5. For your safety and health, please wear lab clothes and disposable gloves throughout the operation.
Instructions for Use：
1. Preparation method for 1 x rapid transfer solution before use:
Please refer to the table below for the preparation of 1L of 1 × rapid film transfer solution:
Western Rapid Transfer Buffer (10X) 100mL
Anhydrous ethanol 200mL
Ultrapure water 700mL
Prepare 5-6 cut filter papers and transfer films. If using PVDF film, soak it in anhydrous methanol for at least 10 seconds before placing it in 1 x rapid transfer solution.
2. Operation steps and film transfer conditions:
① Soak the filter paper and sponge in 1 x rapid film transfer solution, making sure they are completely soaked.
② Rinse the gel in ultrapure water for 2 minutes to remove SDS from the gel surface; Then immerse the gel in 1 × rapid membrane transfer solution.
③ Please note: The rinsing time should not exceed 2 minutes, otherwise proteins with higher molecular weight cannot be completely transferred.
3. Please complete the transfer sandwich structure in the following order:
→ Negative pole (cathode) → sponge → filter paper → gel → transfer film → filter paper → sponge (whether to use according to the thickness of sandwich) → positive pole (anode)
Transfer the sandwich structure to the electrophoresis tank, set a constant current of 400 mA, and complete the protein transfer in 15-35 minutes for electrophoresis

Specifications

Sinónimos

Transfer Buffer (10X)

Especificaciones y pureza

BioReagent, for western blot, sterile-filtered, 10X

Estabilidad y almacenamiento

Store at 2-8°C long term (12 months). Upon receipt, it is recommended to aliquot.

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent, for Western blot, Sterile-filtered

Nombres e identificadores

• PH: 8.2-8.8