Qualitative Identification of Aminotransfer Reactions
Qualitative assays for aminotransfer reactions can be used to: detect the presence of aminotransferases.
Topic
Biochemistry Lab
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
99 resources · protocols, FAQs & articles
Identification of tannins
Tannins, also known as tannins, are a class of complex polyphenolic compounds found in plants. Tannins can be combined with proteins to form an insoluble precip
Identification of saponins
Quantitative determination of saponins can be carried out by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), colorimetric method
Experiment for the determination of total sugar content
(Source: Laboratory instruction in biochemistry and molecular biology, Shao Xueling et al, Wuhan University Press, 2003)
Experiments on the preparation of dispersions
Dispersions are dry powdery preparations of drugs or with suitable excipients made by crushing and uniform mixing for internal or topical use. Internal dispersi
Qualitative identification of volatile oils
Volatile oils, also known as essential oils, in English for essential oils, is a class of aromatic odors present in plants, can be distilled with water vapor an
Residue analysis experiments for organochlorine pesticides
Usually organochlorine pesticides (OCLs) are divided into DDT and its analogues, hexachlorohexane and cyclopentadienyl derivatives, which are not easy to be dec
Effects of pH, temperature, and inhibitors on sucrase activity
Design your own experimental program to determine the effects of pH, temperature and inhibitors on sucrase activity. (Source: Laboratory instruction of biochemi
paper chromatography
Using paper as a carrier, a liquid stationary phase (e.g., water, formamide, or others) is uniformly adsorbed on the paper, and a solvent that is not miscible w
Extraction and isolation of free hydroxyanthraquinone components from Cynodon dactyliflorus
The extraction and isolation of the free hydroxyanthraquinone components in Tiger Balm can be applied to (1) the preparation of drugs and (2) the study of pharm
Methods for the extraction of barmartine and the preparation of fenugreekin
Palmatine is a quaternary ammonium alkaloid that is soluble in water and polar organic solvents (e.g., methanol, ethanol, etc.) so it can be extracted with meth
Experiments on the preparation of casein
Source: Biochemistry and Molecular Biology Laboratory Guide, Shao Xueling et al, Wuhan University Press, 2003.
Balanced salt solution preparation experiment
Source of content: Tissue Culture and Molecular Cytology Techniques. (Beijing Publishing House)
Experiments on the preparation of tonics, Chinese medicine combinations and oral liquids
Combination of Chinese medicines refers to liquid preparations for internal use made of water or other solvents, extracted, purified and concentrated by suitabl
Glucose measurement experiment
Understand the blood glucose determination kit method and master the basic principles and methods of blood glucose enzymatic determination. (Source: Laboratory
Experiments on the preparation of fluidized ointment, extract and decoction
The fluid infusion or infusion refers to the preparation made by extracting herbs with suitable solvents, evaporating some or all of the solvents, and adjusting
How to make glass milk?
Glass milk can be used for the recovery of DNA, this method has a variety of uses, the secondary include: purification of DNA fragments from agarose gel aliquot
Extraction, isolation and characterization of the alkaloids of Pink Proteus vulgaris
Han Fangzhi is the root of Stephania tetrandra S. Mcore, a species of the genus Stephania in the family Fangzhiidae, which is an expectorant, antipyretic and an
Identification of cyanogenic glycosides
Cyanogenic glycosides are mainly glycosides with α-hydroxynitrile. This kind of glycoside is easy to be hydrolyzed by dilute acid and enzyme catalyzed hydrolys
Extraction and characterization of rutin
Rutin (Rutin) is widely present in the plant world, has been found to contain rutin in at least 70 kinds of plants, such as tobacco, acacia flowers, buckwheat a
Experiments on the preparation of solution-type liquid pharmaceuticals
Solution-type liquid pharmaceuticals are liquid pharmaceuticals formed by dispersing small molecules of drugs in a solvent in a molecular or ionic (diameter of
Extraction and isolation of palm leaf anthemisine and preparation of fenugreek ethanol
Extraction and isolation of palmarosa anthelmintic alkaloids and experiments on the preparation of yanhuisuo ethanol can be used for: utilizing it to alleviate
Identification of flavonoid glycosides
Flavonoid glycosides are polyphenolic substances that belong to the flavonoid group. Flavonoids (also known as flavonoids) refers to a series of compounds consi
Gelatin zymography
Gelatin zymography can be used for (1) the determination of gelatinase A (MMP-2) and gelatinase B (MMP-9), (2) the determination of inhibitors of gelatinases, a
Extraction, isolation and characterization of anthraquinones in Rheum palmatum (Rheum palmatum)
Rhubarb is recorded in the Shennong Ben Cao Jing (Classic of the Materia Medica of the Divine Husbandman) and many other documents, and is used for draining the
Identification of Glycosides
Glycosides, also known as ligands, are compounds formed when a sugar or a derivative of a sugar is joined to another type of non-sugar substance through the car
Identification of alkaloids
A class of nitrogen-containing alkaline organic compounds found in nature (mainly in plants, but some are also found in animals) have alkaloid-like properties,
Identification of anthraniloside
Anthracene glycoside and alkali salt chromogenic reaction (Borntrager reaction, sublimation material face up, put under the microscope observation, can see most
Extraction and identification of Diosgeninogenin
In plants Diosgenin is present in combination with glucose and rhamnose to form Dioscin. When extracting and separating, Dioscin is generally hydrolyzed into Di
Identification of cardiac glycosides
The molecule consists of an alcohol group or alcohol-like group (ligand, glycoside or glucoside) bound to a variable number of sugar molecules. If the ligand co
Experiments in the preparation of liquors and tinctures
Liquor refers to the medicinal herbs with distilled liquor extract made of clarified liquid preparations, generally prepared by impregnation, a few percolation
Experiments on the preparation of granules
Granules are dry granular preparations made of drugs or herbal extracts with suitable excipients or fine powder of herbs. Preparation process: treatment of raw
Extraction, isolation and characterization of heptaphylloside and heptaphyllolactone from the skin of Qin Pei
Qinpi is the bark of Fraxinus Chinensis Poxb or F. rhynchophylla Hance or F. bungeana DC, which is bitter in flavor and slightly cold in nature. It is bitter in
Experiments on the effect of reaction time on product formation
In this experiment, sucrose was used as the substrate to determine the time course curve of the reaction between sucrase and the substrate, i.e., under the opti
Monosaccharide fermentation test
The monosaccharide fermentation test can be used to (1) test microorganisms with different abilities to utilize various carbon sources and (2) various tests for
Determination of various pesticide residues in vegetables
In recent years, regarding the detection technology of pesticide residues in food, the United States and the European Union have adopted more multi-residue dete
Assay for the determination of the activity of the enzyme glutamine-alanine transaminase
The basic principle of the determination of glutamine-alpha transaminase activity; master the method of the determination of glutamine-alpha transaminase activi
Determination of amide herbicide residues in cereals and oilseeds
Some herbicides can cause damage to the next crop during application resulting in reduced yields, so residues in agricultural fields have also attracted the att
Experimental determination of macrolide antibiotic residues in fish and meat
Macrolide antibiotics are antibiotics commonly used as veterinary drugs. There are many methods available for the determination of macrolide residues in animal
Residue analysis experiments of carbamate insecticides
Urethane is generally non-corrosive and its storage stability is excellent. Its greatest advantage is that it remains in the plant for only a short time, and mo
Analysis of peroxidase isoenzymes in medicinal plants and comparative activity experiments
In this experiment, polyacrylamide gel electrophoresis was used to analyze and compare the peroxide isoenzymes, and their activities were determined by guaiacol
Experiment for the determination of phenoxyphenoxyacid herbicide residues in plant products
Some herbicides can cause damage to the next crop during application resulting in reduced yields, so residues in agricultural fields have also attracted the att
Protein binding assay with cyanogen bromide-activated agarose
This is an effective method for covalently immobilizing enzymes or proteins, such as peroxidase or antibodies, in agarose or cross-linked dextran. A serious dis
Subchronic toxicity test
Subchronic toxicity (subchronic toxicity) refers to the toxic effects that occur when experimental animals are exposed to a large dose of a foreign compound for
Experimental residue analysis of pyrethroid insecticides
Pyrethroids are an important class of synthetic insecticides with high efficiency, broad-spectrum, low toxicity and easy biodegradation. Source: Food Safety Tes
Detection of Saccharomyces cerevisiae vectors and cloned gene expression products
LacZ fusion vectors were constructed to study yeast gene regulation. Because of the simplicity and sensitivity of this assay, yeast genes are often labeled with
Experimental inhibition of succinate dehydrogenase and malonate
Succinate dehydrogenase is an important enzyme in the tricarboxylic acid cycle, and the determination of the presence or absence of succinate dehydrogenase acti
Extracellular recording experiments in in vivo animals
Source : Practical Laboratory Techniques in Neurobiology
Determination of organophosphorus pesticide residues
In order to study the nature and role of food contaminating factors, some modern techniques are used to detect their levels in food, to determine the safe dose
Experimental determination of nitrofuran metabolite residues in foods of animal origin
Most of the reported methods for the detection of nitrofuran residues still analyze the prodrugs. In recent years, research organizations have begun to focus on
Pretreatment experiments for neuronal cell culture substrates
Almost all neural cell cultures must have macromolecules as growth substrates to which the cells adhere in order to grow. Commonly used are polylysine and polyo
Experimental studies on the structure and biological activity of algal polysaccharides
The main component of algal cell wall and extracellular polysaccharide is mucopolysaccharide, the monosaccharide composition of mucopolysaccharide of a part of
Determination of residues of aminoglycoside antibiotics in experiments
An aminoglycoside is a carbohydrate that contains one or more amino groups in its glycosidic structure. It is an antibiotic often used as a veterinary drug. Sou
Experiment for the determination of residues of eprinomectin, abamectin, doramectin and ivermectin in liver or muscle
The technique of determining the residues of epoetin, abamectin, doramectin and ivermectin in liver or muscle by SPE-HPLC-MS/MS is gradually maturing. Source: F
Multi-residue determination of tetracyclines in foods of animal origin
Tetracyclines (TCs) antibiotics are produced by actinomycetes and all belong to hydrogenated and tetrabenzene ring derivatives in their chemical structure and h
Determination of chloramphenicol and its related compounds residues in animal tissues
There are many reported methods for the determination of chloramphenicol, and among the screening methods, GC-ECD and ELISA are more commonly used. source: Food
Experimental method for the determination of chalcones residues in animal tissues
Quinolone residues in tissues are predominantly prodrugs, so prodrugs are generally chosen as indicator residues. Most of the metabolites are metabolized quickl
Ultraviolet cross-linking experiments of proteins and nucleic acids
Irradiation of protein-nucleic acid complexes with UV light induces covalent bond formation where nucleic acid nucleoproteins are in close contact, thus selecti
Nucleic acid transmission electron microscopy sample preparation experiments
Nucleic acid molecular chain is generally long, the use of ordinary drop or spray method is easy to make its structure damaged. Therefore, at present, most of t
Nippon staining assay
Source : Practical Laboratory Techniques in Neurobiology
Experiments for the determination of trypsin
This presentation describes proteases that preferentially cleave Arg and Lys peptide bonds. Source: Laboratory Manual of Enzymology
LFB myelin staining method
Myelin is a membrane that wraps around the axon of a nerve cell, i.e., myelin consists of the cell membrane of myelinating cells; current research pays attentio
Cultured Cell Membrane Clamp Recording Experiment
Source : Practical Laboratory Techniques in Neurobiology
Nerve tissue block membrane clamp whole-cell recording experiments
Source : Practical Laboratory Techniques in Neurobiology
Tissue thin section membrane clamp whole cell recording experiment
Source : Practical Laboratory Techniques in Neurobiology
Binding experiments with diaminohexyl spacers
Proteins are not directly immobilized to the substrate; spacer molecules can act as bridges connecting proteins to the substrate. Source: Laboratory Manual of E
Periodic acid activation assay of cellulose
This method is suitable for materials with a critical diol structure, such as cellulose, agarose, and dextran. Oxidation of periodate results in the formation o
Experiments on the introduction of sulfur groups in antibodies or proteins
Antibodies or other proteins must have a free thiol group in order to obtain immobilized enzymatic activity. This group can be introduced by specific reagents,
Experimental analysis of herbicide residues in rice
Residues in agricultural fields have also attracted the attention of agrochemists because some herbicides can cause damage to the following crop during applicat
Determination of organochlorine pesticide residues of pyrethroids and polychlorinated biphenyls (PCBs) in brown rice in an experiment
Due to the requirement to detect more and more pesticide species, the limit is getting lower and lower, multi-residue detection technology has become the mainst
Experimental determination of thyroid inhibitor residues in foods of animal origin
Thyroid inhibitors (antithyroida gents) are thioureas, which can be categorized into thioredoxins and imidazoles. Most of the early methods of purification trea
Neurological bundle tracking experiment
Source : Practical Laboratory Techniques in Neurobiology
Intracellular calcium imaging experiments
Source : Practical Laboratory Techniques in Neurobiology
Experimental determination of hormone residues in foods of animal origin
Hormone residues in foods of animal origin may jeopardize the health of consumers, with sex and growth-promoting hormones, which are currently the most used, po
Intracellular recording experiments
Source : Practical Laboratory Techniques in Neurobiology
Preparation of heparin by ion exchange method
Heparin (Heparin) is a class of acidic mucopolysaccharides that are stable to alkali and heat, and exists in organisms as complexes bound to proteins, which are
mRNA isolation assay
Most mRNAs from mammalian cells have a poly(A) tail at the 3' end, so mRNA can be isolated from large amounts of cellular RNA by oligo(dT)-cellulose affinity ch
Experiment on the determination of residues of homotrizoic and substituted urea herbicides in wheat
Determination of residues of homotriazide herbicides and substituted urea herbicides in wheat to prevent some herbicides from causing damage to the next crop du
Spectroscopic determination of cytochrome P-450
Cytochrome P450 (cytochromeP450 or CYP450, for short) is a superfamily of a class of ferrous heme-thiolate proteins that are involved in the metabolism of endog
Experiments on the preparation of sodium bicarbonate and bismuth subcarbonate powder (Xipi's powder II)
Dispersions are dry powdery preparations of drugs or with suitable excipients made by crushing and uniform mixing for internal or topical use. Internal dispersi
Silica G Thin Layer Chromatography of Sugars
(Source: Laboratory instruction in biochemistry and molecular biology, Shao Xueling et al., Wuhan University Press, 2003)
PPO Activity Measurement
PPO activity assay can be applied to (1) study the physiological mechanism and regulation of PPO. (2) To understand the relationship between enzyme activity and
Multi-residue assay of 446 pesticides in fruits and vegetables
China has made great progress in recent years in the series determination of many kinds of pesticide residues. Source: Food Safety Monitoring Technology (Chemic
Determination of 14 β-lactam veterinary drug residues in foodstuffs of animal origin
If veterinary drugs are used inappropriately or without a prescribed rest period, they can lead to excessive residues of veterinary drugs in animal foods, and t
Middle Cerebral Artery Embolism (MCAO) Model in Rats by Wire Embolization Method
Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press
Experimental effects of temperature and pH on salivary amylase activity
This experiment takes the action of salivary amylase on starch at different temperatures and pH as an example to observe the effect of temperature and pH on the
Analysis of DNA-protein interactions with proteins synthesized in vitro from cloned genes
Proteins synthesized in vitro are extremely useful for determining whether a cloned gene encodes a specific DNA-binding protein and for analyzing DNA-protein in
Experimental modeling of epilepsy in lithium-pilocarpine rats
Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press
Base hydrolysis and activation experiments by diindolyl carbonyls
This technique is more efficient and gentle compared to the chloride method. Source: Laboratory Manual of Enzymology
Covalent immobilization of enzymes on non-porous glass surfaces
The resistance of glass to strong acids and solvents and its compact solid structure make it a valuable material for enzyme immobilization in technical processe
Cofactor (NADPH) Solution
NADPH is a coenzyme called reduced coenzyme II, the scientific name of reduced nicotinamide adenine dinucleotide phosphate, once known as pyridine triphosphate
Determination of absorbance values of solutions by spectrophotometer
absorbance
Determination of cytochrome b5
Cytochrome b5 is a cytochrome deposited in the endoplasmic reticulum membrane of higher animals. It can be purified from rabbit liver microsomes. Because it is
Rapid screening and determination of 105 pesticide residues in apple juice concentrate
In recent years, China has made great progress in the series of determination of various pesticide residues, and has issued four national standard methods. For
Determination of 22 sulfonamide residues in foods of animal origin
Sulfonamides (SAs) is a general term for a class of drugs with the structure of p-aminobenzenesulfonamide. They are widely used as chemotherapeutic drugs for th
Experiments in the production of a model of Parkinson's disease
Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press
Cross-linking experiments of proteins with dimethyloctanediimide
This method is representative of various cross-linking reactions and is one of the oldest and most practical. It allows the formation of intermolecular bridges
Experimental determination of b-agonist residues in liver, kidney and meat
Beta-agonists, chemically belonging to the phenylethanolamine group, are chemical analogs of catecholamines, epinephrine and norepinephrine. Source: Food Safety
Experiments for the determination of luciferase
Detection of Firefly Luciferase Activity by Using Luciferin as a Substrate
Related topics
Techniques often explored alongside immunological experiments.