Caspase 3 Mouse mAb - 一次抗体, specific to Human CASP3

Cat. No.: Ab156544
注文可能
グレード&純度 Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
ELISA, Flow Cytometry, IF/ICC, WB
Host species
マウス
Reactive species
マウス, 人間, 助言
Isotype
Mouse IgG2a
Clone Number
CD01/2F2
Conjugation
Unconjugated
★
サイズ
USA
ドイツ (EU)*
価格
個数
10μl
Ab156544-10μl
9 在庫あり
—
$99.90
50μl
Ab156544-50μl
1 在庫あり
—
$259.90
100μl
Ab156544-100μl
1 在庫あり
—
$419.90
1ml
Ab156544-1ml
US製・受注生産 · 2~4週間 ·
—
$3,399.90
Enter a quantity for the sizes you want to add.
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このグレードを選ぶ理由

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated ベースライン干渉を最小限に抑える必要がある、感度の高いクロマトグラフィーおよび分析ワークフロー向け。

🌡

保管・配送

Store at -20°C,Avoid repeated freezing and thawing Ice chest + Ice pads に出荷。正確な仕様については、ロット固有の COA をご確認ください。

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品質書類

SDS、COA、データシート、仕様書をダウンロードできます。ロット固有の COA はロット番号検索で入手可能です。

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文献的裏付け

クロマトグラフィー、有機合成、クロスカップリング反応に関する 0 件の査読済み論文で引用されています。

概要

Mouse anti Human CASP3 Antibody, clone CD01/2F2, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

仕様

Product Name
Caspase 3 Mouse mAb - 一次抗体, specific to Human CASP3
同義語
A830040C14Rik antibody | Apopain antibody | CASP 3 antibody | CASP-3 antibody | CASP3 antibody | CASP3_HUMAN antibody | Casp3a antibody | Caspase 3 antibody | Caspase 3 | Caspase-3 subunit p12 antibody | Caspase3 antibody | CC3 antibody | CPP 32 antibody
仕様と純度
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL
生化学的・生理学的メカニズム
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many protein
宿主種
マウス
特異性
Human CASP3
免疫原
Recombinant protein corresponding to Human CASP3
ポジティブコントロール
WB: Jurkat, HeLa, HEK293, NIH/3T3 and C6 whole cell lysates; Mouse liver tissue lysates Flow Cytometry: Jurkat cells IF/ICC: HeLa cells
活用
Unconjugated
等級
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
ライトチェーン・タイプ
kappa
SDS-PAGE
150.0 KDa
精製方法
Protein A purified
集中
1.0 mg/mL
Product Line
生物製品
保管と配送
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
画像

Caspase 3 Mouse mAb (Ab156544) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab156544) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 34,36 kDa
Exposure time: 16.2 s 

Caspase 3 Mouse mAb (Ab156544) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab156544) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 34 kDa
Exposure time: 120.0 s 

Caspase 3 Mouse mAb (Ab156544) - Flow Cytometry ​
Flow Cytometry analysis of Jurkat cells stained with Caspase 3  Mouse mAb (Ab156544) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2a-kappa, anti-A29L (Ab210599) was used as an isotype control (Blue). ​

Caspase 3 Mouse mAb (Ab156544) - ICC/IF
IF analysis of Caspase 3 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Caspase 3 Mouse mAb (Ab156544) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

適用#テキヨウ#
適用#テキヨウ#
Dilution info
IF/ICC

1/200

WB

1/1000 - 1/200000

Flow Cytometry

1/200 - 1/12500

ドキュメント

📋 安全データシート(SDS)

危険性、取扱い、保管、および規制遵守を網羅した包括的な文書。

<<<<<<< HEAD SDS をダウンロード → ======= SDS をダウンロード → >>>>>>> release248-p3

✅ 分析証明書(COA)

ロット固有の品質データ。ロット番号を入力して、正確な COA を取得してください。

COA を検索 →

📊 データシート

製品仕様と用途のクイックリファレンス概要。

データシートを見る →

🔬 仕様書

このグレードの完全な品質特性と合格基準。

仕様書を見る →

詳細データ

関連ターゲット(人間)
CASP3 Tchem Caspase-3 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
証明書(CoA、COO、BSE/TSEと分析図)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate Type日付商品
ZJ25F0116463ZJ25F0116463Certificate of AnalysisOct 09, 2026 Ab156544
ZJ25F0116464ZJ25F0116464Certificate of AnalysisOct 09, 2026 Ab156544
ZJ25F0116465ZJ25F0116465Certificate of AnalysisOct 09, 2026 Ab156544
よくある質問と記事
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当社のグレード選定ガイドでは、カタログ内のすべてのバリエーションについて、純度、安定剤の状態、用途への適合性を網羅しています。

Carrier Free グレードガイドを見る → Validated グレードガイドを見る → ExactAb™ グレードガイドを見る → Azide Free グレードガイドを見る → High Performance グレードガイドを見る → PBS Only グレードガイドを見る →

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