Caspase 3 Mouse mAb - 一次抗体, specific to Human CASP3

Cat. No.: Ab213595
注文可能
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
ELISA, Flow Cytometry, WB
Host species
マウス
Reactive species
マウス, 人間, 助言
Isotype
Mouse IgA
Clone Number
CD04/3D12
Conjugation
Unconjugated
★
Size
USA
ドイツ (EU)*
Price
Qty
10μl
Ab213595-10μl
5 在庫あり
—
$99.90
50μl
Ab213595-50μl
在庫あり ≥10
—
$259.90
100μl
Ab213595-100μl
3 在庫あり
—
$419.90
1ml
Ab213595-1ml
US製・受注生産 · 2~4週間 ·
—
$3,399.90
Enter a quantity for the sizes you want to add.
🧪
Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

概要

Mouse anti Human CASP3 Antibody, clone CD04/3D12, could be used for WB, Flow Cytometry, ELISA and so on.

Specifications

Product Name
Caspase 3 Mouse mAb - 一次抗体, specific to Human CASP3
同義語
A830040C14Rik antibody | Apopain antibody | CASP 3 antibody | CASP-3 antibody | CASP3 antibody | CASP3_HUMAN antibody | Casp3a antibody | Caspase 3 antibody | Caspase 3 | Caspase-3 subunit p12 antibody | Caspase3 antibody | CC3 antibody | CPP 32 antibody
仕様と純度
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL
生化学的・生理学的メカニズム
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many protein
宿主種
マウス
特異性
Human CASP3
免疫原
Recombinant protein corresponding to Human CASP3
ポジティブコントロール
WB: Jurkat, HeLa, HEK293, NIH/3T3 and C6 whole cell lysates; Mouse liver and Mouse lung tissue lysates Flow Cytometry: Jurkat cells
活用
Unconjugated
等級
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
ライトチェーン・タイプ
lambda
SDS-PAGE
150.0 KDa
精製方法
Protein A purified
集中
1.0 mg/mL
保管と配送
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
画像

Caspase 3 Mouse mAb (Ab213595) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab213595) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 31,34 kDa
Exposure time: 60.0 s

Caspase 3 Mouse mAb (Ab213595) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab213595) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 33 kDa
Exposure time: 60.0 s

aspase 3 Mouse mAb (Ab213595) - Flow Cytometry ​
Flow Cytometry analysis of Jurkat cells stained with Caspase 3  Mouse mAb (Ab213595) (Red) at 1.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

適用#テキヨウ#
適用#テキヨウ#
Dilution info
WB

1/1000 - 1/200000

Flow Cytometry

1/200 - 1/12500

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

関連ターゲット(人間)
CASP3 Tchem Caspase-3 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
証明書(CoA、COO、BSE/TSEと分析図)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
よくある質問と記事
Flow Cytometry Using Methanol Permeabilization
Molecular Composition, Signal Transduction Mechanisms, and Tissue Injury Regulation of the TWEAK-Fn14 Pathway
TNF Signaling Pathway: Receptor Layering Structure, Complex Conversion Mechanisms, and the Inflammation–Death Effector Network
Killer T Cell-Mediated Apoptosis Pathway: Recognition, Effector Molecule Release, and Target Cell Death Mechanisms
Proteolytic Enzyme Networks in Cell Injury: Activation Sequence, Substrate Cleavage, and Experimental Attribution
Applications of the PLSCR Family in Cell Death, Coagulation, and Inflammatory Signaling Research
Necroptosis and Tumor Immunity: The RIPK1–RIPK3–MLKL Axis, ZBP1 Signaling, and Experimental Determination
ADAR1: A Potential Target in Tumor Immunity
ソリューション計算機
レビュー

顧客レビュー

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

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