ARPC4 Antibody - 1차 항체, specific to ARPC4, Rabbit IgG

Cat. No.: Ab089682

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, See COA

Application

IF/ICC, IHC, WB

Host species

토끼

Reactive species

마우스, 인간, 조언

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human ARPC4 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:

WB: 0.01-2µg/mL
IHC: 5-20µg/mL

IF/ICC: 2.3µg/mL
Protein Function
Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. 

Specifications

Product Name

ARPC4 Antibody - 1차 항체, specific to ARPC4, Rabbit IgG

동의어

Actin related protein 2/3 complex subunit 4 20 kD antibody | Actin related protein 2/3 complex subunit 4 20kDa antibody | Actin related protein 2/3 complex, 20-KD subunit antibody | Actin-related protein 2/3 complex subunit 4 antibody | ARC20 antibody | A

사양 및 순도

Carrier Free, ExactAb™, Azide Free, Validated, See COA

숙주 종

토끼

특이성

ARPC4

면역원

Recombinant ARPC4 expressed in E.coli (AA 3-165)

포지티브 제어

WB: HeLa, Jurkat, HEK293, THP-1, NIH/3T3 and C6 cell lysates. IHC: Human liver tissue. IF/ICC: HeLa cells.

변화

Unconjugated

등급

Azide Free, Carrier Free, ExactAb™, Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Antigen affinity purified + Protein A purified

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: ARPC4 Antibody (Ab089682)

ARPC4 Antibody (Ab089682) - Western Blot
All lanes: ARPC4 Antibody (Ab089682) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 20 kDa
Observed band size: 19 kDa
Exposure time: 5.0 s

IHC: ARPC4 Antibody (Ab089682)

ARPC4 Antibody (Ab089682) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 20 µg/mL ARPC4 Antibody (Ab089682)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: ARPC4 Antibody (Ab089682)

ARPC4 Antibody (Ab089682) - IHC
DAB staining on IHC
Sample: Human Liver Tissue
Primary Ab: 20 µg/mL ARPC4 Antibody (Ab089682)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody. 

IF/ICC: ARPC4 Antibody (Ab089682)

ARPC4 Antibody (Ab089682) - IF/ICC
IF analysis of ARPC4 Antibody (Ab089682) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ARPC4 Antibody (Ab089682) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

적용

• 적용: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.01-2 µg/mL ;

• IF/ICC:

  2.3µg/mL