Knowledge base
Protocols, troubleshooting, and answers — all in one place.
Search step-by-step lab protocols, technical articles, and product FAQs written by our scientific team — or browse by what you're trying to do.
Where do you want to start?
Pick a starting point based on what you're trying to do.
Run a protocol
Step-by-step SOPs for techniques like Western blot, ELISA, IHC, PCR, and more.
Browse 3,226 protocols →Troubleshoot an experiment
When something doesn't work — faint bands, high background, no signal — start here.
299 FAQs →Check specs & purity
Grade definitions, purity data, compatibility tables, and tagged-protein guides.
194 spec sheets →Read the latest research
In-depth technical articles on emerging methods, reagent classes, and applications.
1,862 articles →Browse by technique
Jump straight to everything we have on a specific workflow.
Editors' picks this month
Hand-selected by our scientific team — the most useful reads right now.
Company account structure
Company account structure
Immunoprecipitation (IP) lysates and reagents
Immunoprecipitation is a protein purification method. An antibody for the protein of interest is incubated with a cell extract, allowing it to bind to the protein in solution. The antibody/antigen complex is then isolated from the sample using protein A/G-coupled agarose beads. This separates the protein of interest from the rest of the sample, which can then be analyzed by SDS-PAGE for western blotting.
His-Tag Protein Purification: Common Troubleshooting Questions and Practical Solutions
His-Tag Protein Purification: Common Troubleshooting Questions and Practical Solutions
All categories
The full library, organized by content type.
Technical articles
1862Stay informed with Aladdin Scientific’s collection of technical articles. Discover the latest advancements, methodologies, and insights in scientific research.
- Small-Molecule InhibitorsBreast Cancer-Related Small-Molecule Inhibitors and Their Mechanisms of Action
- EpigeneticsRegulatory Mechanisms of 5mC DNA Methylation
- Lipid metabolismAre Lipids Fats? Understanding the Multiple Functions of Lipids from Cellular Metabolism to mRNA Delivery
- Targeted protein degradationAUTAC or AUTOTAC? Mechanistic Differences Between First- and Second-Generation Autophagy-Targeting Degradation Technologies
- ICP-OESPrinciples, Method Comparison, and Application Selection for Calcium Detection: Analysis of Total Calcium, Ionized Calcium, and Intracellular Ca²⁺
Protocols
3226Access a comprehensive library of scientific protocols and procedures at Aladdin Scientific. Our step-by-step guides help you achieve optimal results in your research projects.
- β-Propiolactoneβ-Propiolactone (BPL) Viral Inactivation — Standard Operating Procedure (SOP)
- MSCsStandard Operating Procedure for Adipogenic Differentiation of Mesenchymal Stem Cells and Evaluation by Oil Red O Staining
- Other experimentsExperimental purification of λ phage particles by precipitation/centrifugation
- Mesenchymal stem cellsStandard Operating Procedure for Chondrogenic Differentiation of Mesenchymal Stem Cells and Evaluation by Alcian Blue Staining
- DNA experimentExperimental preparation of λ phage particles from large-scale lysates
FAQs
299Find quick answers to common queries about Aladdin Scientific’s products, services, and policies in our Frequently Asked Questions (FAQs) section.
- Peptide PurityDetermination of Peptide Purity
- Meet AldaMeet Alda, Aladdin Scientific’s Digital Product Assistant
- Flow-through AntibodiesIs the mouse Fc blocker in μg packaging and is it powdery in nature? Does it dissolve in pbs?
- Flow-through AntibodiesWhat is the difference between our Flow Antibodies available in Test packages and μg packages?
- proteinHow many cells are lysed in 100μl of RIPA protein lysate?
Help & Customer Service
7Visit Aladdin Scientific’s Help & Customer Service section for general inquiries, order assistance, and customer support. Our team is ready to assist with your needs.
Specifications, Grading and Purity
194Explore the detailed specifications, grading criteria, and purity levels of Aladdin Scientific’s biochemicals and reagents. Ensure reliability and performance in your scientific research.
- GradeWhat Grade Is Purum? From Grade Meaning and Specification Interpretation to Reagent Selection
- Grade“Tissue Engineering Grade” Reagents and Materials: Grade Meaning, Critical Quality Attributes, and Selection Methods
- COAReagent Purity Labeling System and Interpretation Principles
- Electronic GradeFrom EL to UPSSS: Cleanliness Grades, Key Specifications, and Selection Logic for Electronic-Grade Reagents
- AVI tagAVI-Tag Grade Reagents
Order, shipping & account help
Non-scientific questions — shipping times, returns, payment methods, and account settings. Our customer service team is one click away.
Talk to a scientist.
Our scientific support team includes PhDs in molecular biology, chemistry, and cell biology. Typical response time: under 4 hours.