Avidin from egg white, CAS No.1405-69-2

CAS: 1405-69-2 Cat. No.: A103033 EC 번호: 215-783-6

등급 & 순도 Bioactive, Native, ≥98%(SDS-PAGE), ≥10 U/mg solid; >10 U/mg protein; ≥0.9 mg protein/mg solid (see COA for assay result)

Accession #

P02701

Bioactivity

≥10 U/mg solid; >10 U/mg protein

개요

Avidin is a tetrameric protein. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer) each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.

Avidin's affinity for biotin is exploited in wide ranging biochemical assays, including Western Blot, ELISA, ELISPOT and pull-down assays. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support.

Avidin and biotin are routinely conjugated to indicator enzymes, such as alkaline phosphatase and horseradish peroxidase for use in diagnostic kit development and manufacturing.

Application：

Avidin from egg white can be used in assays for functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.

사양

Product Name

Avidin from egg white, CAS No.1405-69-2

동의어

Avidin from egg white | Avidin, chiken egg white | Avidin, Egg White | Anti-biotin protein | Immunopure Avidin | AVD

등급

Bioactive, Native

사양 및 순도

Bioactive, Native, ≥98%(SDS-PAGE), ≥10 U/mg solid; >10 U/mg protein; ≥0.9 mg protein/mg solid (see COA for assay result)

생화학 및 생리적 메커니즘

The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin). Post-translational: N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)

생체 활성

≥10 U/mg solid; >10 U/mg protein

가입 번호

P02701

예상 분자량

64,000 (tetramer of 16,000 MW subunits)

CAS

1405-69-2

분자 유형

Protein

Product Line

생물 제품

보관 및 배송

집중력

≥10 U/mg solid; >10 U/mg protein; ≥0.9 mg protein/mg solid (see COA for assay result)

재구성

Reconstitute in 0.1 M phosphate buffer, pH 6.8.

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at -20℃ long term (36 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

단위 정의

One unit will bind one microgram of d-biotin.