BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Room temperature Ships Normal Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Benedict‘s Reagent, also known as Benedict’s Solution, is a light blue chemical reagent named after the American chemist Stanley Benedict. Its preparation principle is as follows: when copper sulfate solution is slowly added to an alkaline solution composed of sodium citrate and anhydrous sodium carbonate, sodium citrate acts as a hydrophilic masking agent, forming a soluble complex salt with copper ions. This effectively prevents the precipitation of copper hydroxide, maintaining the solution in a stable, clear light blue state. Detection Principle Under alkaline heating conditions, reducing sugars (including most monosaccharides and disaccharides except sucrose) reduce Cu²⁺ in Benedict‘s Reagent to Cu⁺. The Cu⁺ then combines with OH⁻ to form yellow copper hydroxide (CuOH), which upon heating is rapidly converted to a brick-red cuprous oxide (Cu₂O) precipitate. By observing the color change of the precipitate, the presence and approximate content of reducing sugars in the sample can be determined qualitatively or semi-quantitatively.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View BioReagent grade guide →
