Carboxypeptidase B, also known as peptidyl - L - lysine (L - arginine) hydrolase and arginase, is a metalloprotease that can specifically hydrolyze basic amino acids (lysine, arginine, histidine) at the C - terminus of proteins.
This product is a recombinant carboxypeptidase B lyophilized powder obtained by isolation and purification from recombinant Pichia pastoris, followed by freeze - drying. It is a biochemical - grade genetic engineering enzyme, free from exogenous virus contamination, and does not contain enzyme inhibitors such as DFP, PMSF, and TLCK. It has the same activity and specificity as natural carboxypeptidase B.
Product Advantages
Animal - free origin: No animal viruses, no pathogenic substances, no exogenous factor contamination, and high safety.
High purity and high specific activity.
Production scale is over 1000L.
Compliance: Production equipment and production environment meet relevant regulatory requirements and conform to GMP guidelines.
Complete quality documents: Relevant regulatory support documents can be provided according to customer needs.
Product Characteristics
Appearance: White or off - white powder
Enzyme specific activity: ≥150 U/mg protein
Molecular weight: 36kD ± 3.6kD
Purity: ≥90% (SDS - PAGE)
Protein content: ≥30%
Optimal pH: 7.0 - 9.0
Solubility: Soluble in buffer solution
Recommended Usage Methods
Preparation of protein
Dissolve the protein in a 25mM Tris - HCl solution with pH 7.6.
Preparation of enzyme solution
Weigh the required enzyme powder and dissolve it in 25mM Tris - HCl (pH 7.6) to make the enzyme solution concentration 1 - 10mg/ml.
Reaction
Recommended enzyme amount: Target protein (mass ratio) = 1:50 - 1:1000, and the optimal pH is 7.0 - 9.0.
Product Applications
Production of various proteins or polypeptides, such as insulin and its analogs, beauty peptides, antimicrobial peptides, food flavor peptides, etc.
Quality detection of antibodies.
Determination of amino acids at the C - terminus of proteins.
Storage stability
The lyophilized powder can be stably stored for at least 24 months at -15°C or below. After being dissolved in 25 mM Tris-HCl (pH 7.6) and stored at -15°C or below, there is no loss of activity even after 10 cycles of repeated freezing and thawing. It is recommended that the dissolved product be stored at 2–8°C for no more than 7 days, and at -15°C or below for no more than 30 days.
Precautions and Disclaimer
Its activity is competitively inhibited by arginine and lysine; metal ion chelators (such as EDTA) also have an inhibitory effect on the enzyme activity.
This product is hygroscopic and should be equilibrated at room temperature before being taken and used.
This product is for research use only and shall not be used for the diagnosis or treatment of animals or humans.
K2517810