등급 & 순도ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.Ex:650nm?Fluorescence excitation maximum at 650 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal.Em:660nm?Fluorescence emission maximum at 660 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk.5μL/test
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
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CD25 Rat mAb (APC) (Ab179012) - Flow Cytometry BALB/c mouse splenocytes are stained with CD25 Rat mAb (APC) (Ab179012) and CD4 Rat mAb (FITC) (Ab181111) (right). BALB/c mouse splenocytes are stained with CD4 Rat mAb (FITC) (Ab181111) and Rat IgG Isotype Control Antibody (APC) (Ab180313) (left).
CD25 Rat mAb (APC) (Ab179012) - Flow Cytometry Con A-stimulated (1 day) BALB/c mouse splenocytes (right panel) stained with CD25 Rat mAb (APC) (Ab179012) (red) compared to an isotype control (blue). Blue - Rat IgG Isotype Control Antibody (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody. Untreated BALB/c mouse splenocytes were used as control (left panel).
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Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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