등급 & 순도ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
ExactAb™, Validated, 1.0 mg/mL ExactAb™,Validated 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
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보관 및 배송
Store at -20°C,Avoid repeated freezing and thawing Ice chest + Ice pads 출고. 정확한 사양은 로트별 COA를 확인하세요.
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품질 문서
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
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문헌적 근거
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
개요
Rabbit anti Human CDKN3 Antibody, Polyclonal, could be used for WB, IHC and so on. Application
WB: 1/500-1/2000 IHC: 1/100-1/300
IF/ICC: 1/200 Protein Function May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner.
사양
Product Name
CDKN3 Antibody - 1차 항체, specific to CDKN3, Rabbit IgG
A synthetic peptide derived from human CDKN3 (AA 31-80).
포지티브 제어
WB: HEK293, Hep G2, U-2 OS, NIH/3T3 and C6 cell lysates. IHC: Human colon cancer tissue. IF/ICC: HeLa cells.
변화
Unconjugated
등급
ExactAb™, Validated
아이소타입
Rabbit IgG
SDS-PAGE
150 kDa
정화 방법
Immunogen affinity purified
집중력
1.0 mg/mL
Product Line
생물 제품
보관 및 배송
모양
Liquid
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
CDKN3 Antibody (Ab095865) - Western Blot All lanes: CDKN3 Antibody (Ab095865) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 18, 24 kDa Exposure time: 4.5 s
CDKN3 Antibody (Ab095865) - IHC Immunohistochemical analysis of paraffin-embedded human Colon cancer. CDKN3 Antibody (Ab095865) was diluted at 1/200 (4° overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min).
CDKN3 Antibody (Ab095865) - IF/ICC IF analysis of CDKN3 Antibody (Ab095865) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CDKN3 Antibody (Ab095865) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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