GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
개요
Mouse Monoclonal (2G1D5) to CHK1, could be used for WB and ELISA. Application: WB: 1/500 - 1/2000 ELISA: 1/10000
Protein Function: CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.
Purified recombinant fragment of CHK1 (AA: 207-421) expressed in E.coli
포지티브 제어
WB: A431, Hela, NIH/3T3 and K562 cell lysate. HEK293T cells transfected with pCMV6-ENTRY CHK1 cDNA.
변화
Unconjugated
등급
Carrier Free, ExactAb™, Validated
아이소타입
IgG1
라이트 체인 유형
kappa
SDS-PAGE
150 kDa
정화 방법
Protein G purified
집중력
1.0 mg/mL
보관 및 배송
모양
Liquid
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C. Avoid freeze/thaw cycle.
이미지
Chk1 Mouse mAb (Ab096310) - Western Blot All lanes: Chk1 Mouse mAb (Ab096310) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 56 kDa Observed band size: 56 kDa
Chk1 Mouse mAb (Ab096310) - Western Blot All lanes: Chk1 Mouse mAb (Ab096310) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 56 kDa Observed band size: 56 kDa
적용
적용
Dilution info
WB
1/500-1/2000
ELISA
1/10000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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