BioReagent BioReagent 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
Store at 2-8°C Wet ice 출고. 정확한 사양은 로트별 COA를 확인하세요.
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
Plasma total cholesterol consists of two-thirds cholesterol esters and one-third free cholesterol. It is a key component of blood lipids, and both forms are closely associated with cardiovascular and cerebrovascular diseases as well as various pathological conditions. This kit employs the total cholesterol and free cholesterol detection methods recommended by the World Health Organization (WHO), the U.S. Food and Drug Administration (FDA), and China's National Clinical Laboratory Operating Procedures, offering high sensitivity with a detection range of 20–5000 µmol/L, as well as excellent reliability and reproducibility. The kit utilizes the CHOD-PAP endpoint method combined with the classic GPO Trinder enzymatic reaction. The principle is as follows: (1) Cholesterol esterase hydrolyzes cholesterol esters into free cholesterol; (2) Cholesterol oxidase oxidizes free cholesterol to produce hydrogen peroxide; (3) Under the catalysis of peroxidase, the chromogenic substrate is converted into benzoquinone imine, with the optical density being proportional to the cholesterol concentration; (4) The total cholesterol concentration and free cholesterol concentration are calculated separately, and the difference between the two values represents the cholesterol ester concentration.
위험성, 취급, 보관 및 규제 준수를 포괄하는 종합 문서.
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