BioReagent BioReagent 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
Store at 2-8°C Wet ice 출고. 정확한 사양은 로트별 COA를 확인하세요.
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
In solid tissues and cells, cholesterol esters are not only components of the cell plasma membrane but also major constituents of intracellular lipid droplets. Excessive intracellular cholesterol accumulation is closely associated with the formation of atherosclerotic foam cells. Cholesterol determination in solid tissues and cells is far more complex than that in blood. This kit eliminates the need for toxic organic solvent extraction, cumbersome nitrogen drying, and lipid reconstitution steps. It employs high‑efficiency reagents for cell lysis and cholesterol extraction, with optimized enzymatic reactions and procedures, making it simple and easy to use while offering high sensitivity and a linear range of 20–5000 µmol/L. This kit utilizes the enzymatic method recommended by the WHO and China’s National Clinical Laboratory Operating Procedures, combined with the classic GPO Trinder enzymatic reaction. The principle is as follows: (1) Cholesterol esterase hydrolyzes cholesterol esters into free cholesterol; (2) Cholesterol oxidase oxidizes free cholesterol, producing hydrogen peroxide during the reaction; (3) A chromogenic reaction is catalyzed by peroxidase, producing a product with a maximum absorption peak at 550 nm, where the optical density is proportional to the cholesterol concentration; (4) The total cholesterol concentration and free cholesterol concentration are calculated separately, and the difference between the two values represents the cholesterol ester concentration.
위험성, 취급, 보관 및 규제 준수를 포괄하는 종합 문서.
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