CNKR2 Antibody - 1차 항체, specific to CNKSR2, Rabbit IgG

Cat. No.: Ab097024

등급 & 순도 Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, WB

Host species

토끼

Reactive species

마우스, 인간, 조언

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human CNKR2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/100-1/200
Relevance
CNKR2 may function as an adapter protein or regulator of Ras signaling pathways.

사양

Product Name

CNKR2 Antibody - 1차 항체, specific to CNKSR2, Rabbit IgG

동의어

CNKSR2 antibody | CNK2 antibody | Connector enhancer of kinase suppressor of Ras 2 antibody | Connector enhancer of KSR2 antibody | KIAA0902 antibody | KSR2 antibody | RP11 450P7.2 antibody | CNKSR2 | CNK2 | KSR2 | MAGUIN | MRXSHG

사양 및 순도

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

숙주 종

토끼

특이성

CNKSR2

면역원

A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CNK2.

포지티브 제어

WB: MCF7, HUVEC and SH-SY5Y cell lysates. IHC: Human breast cancer tissue.

변화

Unconjugated

등급

Carrier Free, ExactAb™, Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Immunogen affinity purified

집중력

1.0 mg/mL

Product Line

생물 제품

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: CNKR2 Antibody (Ab097024)

CNKR2 Antibody (Ab097024) - Western Blot
All lanes: CNKR2 Antibody (Ab097024) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 117 kDa
Observed band size: 110, 89 kDa
CNKR2 Antibody (Ab097024) recognizes two isoforms of CNKR2.
Exposure time: 303.3 s

IHC: CNKR2 Antibody (Ab097024)

CNKR2 Antibody (Ab097024) - IHC
Immunohistochemical analysis of CNK2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with CNKR2 Antibody (Ab097024) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

적용

적용

Dilution info

IHC

1/100 - 1/200

WB

1/500-1/1000

관련 대상(인간)

CNKSR2 Tbio Connector enhancer of kinase suppressor of ras 2 (0 Activities)

Discover Target: CNKSR2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘

자주 묻는 질문

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CNKR2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.