Coelenterazine 400a - Suitable for molecular biology, ≥95%(TLC) , CAS No.70217-82-2

CAS: 70217-82-2 Cat. No.: C1375502 분자식: C₂₆H₂₁N₃O 분자량: 391.46

GRADE & PURITY Suitable for molecular biology, ≥95%(TLC)

Synonyms

8-Dibenzyl-6-phenyl-imidazo[1 | 2a]pyrazin-3-(7H)-one

Storage

Protected from light,Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

개요

Coelenterazine 400a is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission.
Note: It is not recommended to store dissolved coelenterazine 400a at -20°C or -70°C. Coelenterazine 400a is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.

Specifications

동의어

8-Dibenzyl-6-phenyl-imidazo[1 | 2a]pyrazin-3-(7H)-one

사양 및 순도

Suitable for molecular biology, ≥95%(TLC)

보관 조건

Protected from light,Store at -20°C,Argon charged

배송

Ice chest + Ice pads

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등급

Suitable for molecular biology

순수함

≥95%(TLC)

이름과 식별자

• 정식 스마일: C1=CC=C(C=C1)CC2=C(N3C=C(N=C(C3=N2)CC4=CC=CC=C4)C5=CC=CC=C5)O
• IUPAC Name: 2,8-dibenzyl-6-phenylimidazo[1,2-a]pyrazin-3-ol
• InChIKey: XNNYOKUWNAAJQW-UHFFFAOYSA-N
• INCHI: 1S/C26H21N3O/c30-26-23(17-20-12-6-2-7-13-20)28-25-22(16-19-10-4-1-5-11-19)27-24(18-29(25)26)21-14-8-3-9-15-21/h1-15,18,30H,16-17H2
• 이성체 SMILES: C1=CC=C(C=C1)CC2=C(N3C=C(N=C(C3=N2)CC4=CC=CC=C4)C5=CC=CC=C5)O
• 분자량: 391.46
• Reaxy-Rn: 36019358
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=36019358&ln=

화학 및 물리적 특성

• 용해성: Soluble in methanol or ethanol (NOT in DMSO).
• 굴절률: n20D1.66 (Predicted)
• 끓는점(°C): 551.39° C at 760 mmHg (Predicted)
• 녹는점(°C): 94-98° C
• 분자량: 391.500 g/mol
• XLogP3: 6.200
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 3
• Rotatable Bond Count: 5
• Exact Mass: 391.168 Da
• Monoisotopic Mass: 391.168 Da
• Topological Polar Surface Area: 50.400 Ų
• Heavy Atom Count: 30
• Formal Charge: 0
• Complexity: 524.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

참고 문헌

1. Liu Wang, Yangyi Liu, Jing Sun, Juanjuan Su, Jing Feng, Li Miao, Weijie Zhao, Hongjie Zhang, Kai Liu.  (2025)  In Situ Ultrasound-Triggered Bioluminescence for Combined Sono/Photodynamic Immunotherapy.  ACS Nano,      [PMID:40671351] [10.1021/acsnano.5c06999]

자주 묻는 질문

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by TLC. Lot-specific values are stated on the Certificate of Analysis.

What does Suitable for molecular biology mean?

Suitable for molecular biology indicates that the product has been processed and tested for molecular biology and proteomics. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.

How should this product be stored?

Store at ?20 °C, protected from light, under argon. It is supplied under an argon blanket; reseal under inert gas after each use.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 70217-82-2, the molecular formula is C26H21N3O, and the molecular weight is 391.46 g/mol. InChIKey XNNYOKUWNAAJQW-UHFFFAOYSA-N.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.