COX2 Antibody - 1차 항체, specific to COX2 / Cyclooxygenase 2, Rabbit IgG

Cat. No.: Ab097432

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

토끼

Reactive species

마우스, 인간

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human COX2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-5 µg/mL
IHC: 5-20 µg/mL
Protein Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.

Specifications

Product Name

COX2 Antibody - 1차 항체, specific to COX2 / Cyclooxygenase 2, Rabbit IgG

동의어

COX 2 antibody | COX-2 antibody | COX2 antibody | Cyclooxygenase 2 antibody | Cyclooxygenase 2b antibody | Cyclooxygenase antibody | Cyclooxygenase-2 antibody | Cyclooxygenase2 antibody | EC 1.14.99.1 antibody | fj02a10 antibody | Glucocorticoid-regulated

사양 및 순도

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

숙주 종

토끼

특이성

COX2 / Cyclooxygenase 2

면역원

Recombinant COX2 expressed in E.coli (AA 187-425)

포지티브 제어

WB: RAW264.7 treated with LPS cell lysates. IHC: Human liver, breast cancer, pancreatic cancer and kidney tissues. IF/ICC: HeLa cells.

변화

Unconjugated

등급

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Antigen affinity purified and Protein A purified

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: COX2 Antibody (Ab097432)

COX2 Antibody (Ab097432) - Western Blot
All lanes: COX2 Antibody (Ab097432) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 74 kDa
Observed band size: 71 kDa
Exposure time: 0.5 s

IHC: COX2 Antibody (Ab097432)

COX2 Antibody (Ab097432) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL COX2 Antibody (Ab097432)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: COX2 Antibody (Ab097432)

COX2 Antibody (Ab097432) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL COX2 Antibody (Ab097432)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: COX2 Antibody (Ab097432)

COX2 Antibody (Ab097432) - IHC
DAB staining on IHC
Samples: Human Pancreatic cancer Tissue
Primary Ab: 10 µg/mL COX2 Antibody (Ab097432)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: COX2 Antibody (Ab097432)

COX2 Antibody (Ab097432) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL COX2 Antibody (Ab097432)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IF/ICC: COX2 Antibody (Ab097432)

COX2 Antibody (Ab097432) - IF/ICC 

IF analysis of COX2 Antibody (Ab097432) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with COX2 Antibody (Ab097432) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

적용

• 적용: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.01-5 µg/mL;

• IF/ICC:

  2.5 µg/mL ;

관련 대상(인간)

PTGS2 Tclin Prostaglandin G/H synthase 2 (0 Activities)

Discover Target: PTGS2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PTGS2 Tclin Cyclooxygenase-2 (13999 Activities)

Discover Target: PTGS2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PTGS2 Tclin Cyclooxygenase (1258 Activities)

Discover Target: PTGS2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PTGS2 Tclin Prostaglandin E synthase/G/H synthase 2 (7 Activities)

Discover Target: PTGS2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

작용 메커니즘

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (COX2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.