Crystal Violet Staining Solution Kit - BioReagent,for cell culture,for microscopy

Cat. No.: C1506003

GRADE & PURITY BioReagent, for Microscopy, for Cell culture

Synonyms

Crystal Violet Staining Kit

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

Cell Staining

개요

  Crystal Violet Staining Solution is a commonly used staining solution in tissue or cell staining that can stain cell nuclei dark purple. As a basic dye, crystal violet can bind to DNA in the cell nucleus, thereby achieving nuclear staining.

C1506003	Component	50T	Storage
C1506003A	Crystal Violet Staining Solution	50 mL	2-8℃. Store in the dark.
C1506003B	Toning Solution	50 mL	2-8℃
C1506003C	Mounting Medium	5 mL	2-8℃

Operating Procedures:

Sample Processing:

a) Paraffin Sections:

1. Dewax in xylene for 5–10 minutes.

2. Replace with fresh xylene and dewax again for 5–10 minutes.

3. Immerse in absolute ethanol for 5 minutes.

4. Immerse in 90% ethanol for 2 minutes.

5. Immerse in 70% ethanol for 2 minutes.

6. Rinse with PBS for 2 minutes, repeated 3 times.

7. Stain with Crystal Violet Staining Solution for 10 minutes.

8. Mount the section with Solution C.

Note: If the staining color is too light, extend the staining time. If the staining color is too dark, rinse with Solution B (30 seconds to 1 minute each time), followed by rinsing with water.

b) Frozen Sections:

1. Fix with 4% paraformaldehyde for 30 minutes.

2. Rinse with PBS 3 times, 5 minutes each time.

3. Stain with Crystal Violet Staining Solution for 10 minutes.

4. Mount the section with Solution C.

Note: If the staining color is too light, extend the staining time. If the staining color is too dark, rinse with Solution B (30 seconds to 1 minute each time), followed by rinsing with water.

c) Cultured Cells:

1. Fix with 4% paraformaldehyde for 10 minutes.

2. Rinse with PBS 3 times, 5 minutes each time.

3. Stain with Crystal Violet Staining Solution for 10 minutes.

4. Mount with Solution C.

Note: If the staining color is too light, extend the staining time. If the staining color is too dark, rinse with Solution B (30 seconds to 1 minute each time), followed by rinsing with water.

Precautions:

1. When using this reagent for the first time, it is recommended to take 1-2 samples for a preliminary experiment to determine an optimal staining time.

2. For your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications

동의어

Crystal Violet Staining Kit

사양 및 순도

BioReagent,for cell culture,for microscopy

안정성 및 스토리지

Each component has a shelf life of 1 year under corresponding storage conditions.

보관 조건

Store at 2-8°C

배송

Wet ice

이 제품은 콜드 체인 배송이 필요합니다.지상 및 기타 경제 서비스는 사용할 수 없습니다.

등급

BioReagent, 세포 배양용, 현미경용

자주 묻는 질문

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

What does for microscopy mean?

for microscopy indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.