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Application Protocols
Item-specific validated applications, recommended dilutions, antigen retrieval conditions, incubation times, and positive control samples are not provided in the Product Data for SKU Ab100149. Please consult the CoA/Spec Sheet or contact technical support with lot information for guidance.
General best practices (non-validated, for planning only)
Include appropriate negative/positive controls and isotype controls.
Titrate antibody and optimize blocking, washing, and secondary detection parameters for your specific samples.
Document lot number and storage history to aid reproducibility.
If using HRP-based detection, verify whether the formulation is azide-free (not specified for this item).
Biological Roles
Target overview (literature; not product-specific claims)
DNAJB5 (DnaJ heat shock protein family [Hsp40] member B5) is a J-domain co-chaperone that partners with Hsp70s to regulate ATPase activity and client protein binding/release cycles. As part of the Hsp40/Hsp70 axis, it contributes to protein quality control, especially during cellular stress.
J-domain proteins typically specify client selection and subcellular localization for Hsp70 machinery; DNAJB5 is reported in cytosolic/nuclear compartments depending on context.
Functional context
In proteostasis, DNAJB5 can recruit misfolded or nascent polypeptides to Hsp70 for refolding or triage to degradation pathways (e.g., ubiquitin–proteasome system), thereby limiting aggregation (literature).
Heat shock and other stresses often modulate expression of Hsp40 family members; DNAJB5 expression patterns may be cell type– and stimulus-dependent (literature).
Potential interaction network: Hsp70 family (HSPA proteins) and co-chaperones; specific partners and post-translational modifications should be verified in the relevant model system.
Research utility
Antibodies against DNAJB5 enable monitoring of stress responses, proteostasis perturbations, and chaperone–client interactions in biochemical and cell biological assays.
Note
Species reactivity and epitope of this specific clone are not specified in the Product Data and should be confirmed against the CoA/Spec Sheet before experimental design.
Buffer Applications
Not typically applicable
This product is an antibody reagent and is not used as a buffering agent. For experimental use, prepare suitable antibody diluents (e.g., PBS/TBS-based) as described in Solvent Selection.
For buffer recipes, blocking solutions, and wash conditions, consult your assay protocol and optimize per sample type.
Green Alternatives
Context
Antibodies are aqueous bioreagents; “green” considerations center on formulation, packaging, cold-chain logistics, and waste handling rather than solvent replacement.
Greener practice suggestions (general)
Consolidate orders and choose appropriate pack sizes to minimize shipping frequency and dry-ice/ice-pack use.
Aliquot upon first thaw to avoid repeated freeze–thaw and product waste.
Prefer reusable cold-chain shippers where available; recycle gel packs and insulation consistent with local regulations.
Use azide-free or low-toxicity preservatives when compatible with your application and biosafety requirements (composition for this item is not specified).
Implement miniaturized assay formats (e.g., microfluidic Westerns, 96–384 well ELISA) to reduce reagent consumption and waste.
Trade-offs
Lower preservative content may shorten bench stability; balance environmental considerations with data quality and biosafety.
Room-temperature-stable formulations can reduce cold-chain impact but may not be suitable for all antibody clones.
Pharmaceutical Uses
Scope
This product is designated For research use only (from Product Data) and is not intended for diagnostic or therapeutic use.
Formulation/manufacturing context (general)
Research-grade monoclonal antibodies are commonly used as analytical reagents to characterize process intermediates, monitor protein expression, or qualify cell banks in preclinical R&D environments.
Excipient systems in research antibodies may include stabilizers and preservatives; for this item, no formulation details are specified. If use in regulated environments is contemplated, verify compliance (e.g., USP/Ph. Eur. specifications) and source appropriate GMP-grade reagents.
Documentation
No pharmacopeial status, excipient role, or clinical application is specified for this item; refer to CoA/Spec Sheet and institutional quality requirements.
Physical Properties
Item-specific (from Product Data)
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Concentration/formulation: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
Literature/general values for context (not item specifications)
Isoelectric point (pI): Mouse IgG subclasses commonly span pI ~6–9 (literature), clone-dependent.
Solubility: Readily soluble in aqueous buffers (PBS/TBS) at neutral pH; sensitive to extremes of pH and high shear.
Stability enhancers often used in antibody formulations: carrier proteins (e.g., BSA), sugars (e.g., trehalose), and antimicrobial preservatives (e.g., sodium azide) — presence and amounts are not specified for this item.
Denaturation temperatures: Onset typically ~60–70 °C for IgG (literature), formulation dependent.
Practical notes (general)
Avoid repeated freeze–thaw to prevent aggregation.
Gentle mixing (inversion) preferred over vigorous vortexing.
Filter sterilization (0.22 µm) can reduce particulates if compatible with concentration and formulation.
Quality and Grades
Item-specific quality statements
Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
Clone/isotype, host/reactivity, validated applications, and lot-specific titer: Not specified for this item; refer to CoA/Spec Sheet.
How to interpret antibody quality (general guidance)
Monoclonal antibodies (mAb) provide epitope-specific recognition with reduced lot-to-lot variability versus polyclonals.
Common QC metrics include: SDS-PAGE purity profile, endotoxin level (EU/mg), aggregate content (SEC), binding specificity by WB/IHC/IF/IP/ELISA, and isotype determination. None are specified here for this item.
Chromatography grade/ELISA grade/IHC grade labels, when provided, denote application-focused QC and low background performance; absence here means details are not specified and should be confirmed with the CoA.
Stabilizers/preservatives
Presence/absence and levels of BSA, glycerol, trehalose, or sodium azide significantly affect application compatibility (e.g., MS, live-cell staining, HRP-based amplification). For this product, formulation details are not specified; check label/CoA.
Documentation
For lot release criteria, recommended dilutions, and performance data, consult the CoA/Spec Sheet or contact technical support with SKU Ab100149.
Reaction and Applications
Item-specific tested applications
Manufacturer-validated applications are not provided in the Product Data for SKU Ab100149; consult CoA/Spec Sheet.
General research applications for anti-DNAJB5 mouse monoclonal antibodies (literature/general guidance; not product-specific claims)
Western blotting (WB): Detection of DNAJB5 under denaturing conditions to assess expression across tissues, stress treatments, or cell lines.
Immunofluorescence (IF)/Immunocytochemistry (ICC): Localization studies to visualize DNAJB5 distribution within cells (cytosolic/nuclear patterns may depend on cell type and stress state).
Immunohistochemistry (IHC): Comparative expression in tissue sections; requires clone-specific validation and antigen retrieval optimization.
Immunoprecipitation (IP)/Co-IP: Pull-down of DNAJB5 and associated Hsp70 complexes to study chaperone interactions.
ELISA: Plate-based quantification in lysates or fractions when compatible with capture/detection formats.
Practical tips (general)
Optimize antibody dilution, incubation time, and blocking conditions empirically per sample type.
Include isotype controls and secondary-only controls to evaluate background.
Validate specificity using siRNA/CRISPR knockdown/knockout or overexpression of DNAJB5 where feasible.
For phospho- or isoform-specific questions, ensure the clone’s epitope matches the research objective (epitope not specified for this item).
Reaction Conditions
Not applicable
Chemical reaction conditions (solvents, catalysts, yields) are not relevant to antibody products. For biological assay conditions (e.g., incubation times, temperatures), refer to validated protocols provided with the specific lot. No application conditions are specified in the Product Data for this item.
Safety and Handling
Item-specific hazard information (from Product Data)
GHS classification, Signal word, H-statements, Pictograms: Not specified for this item; refer to SDS.
Storage and shipping (from Product Data)
Storage: Store at −20 °C; avoid repeated freezing and thawing.
Shipping: Ice chest + ice pads.
General laboratory safety guidance for protein antibodies (literature/good practice)
PPE: Lab coat, gloves, and safety glasses. Handle as a protein biological reagent; avoid ingestion/skin contact.
Biosafety: Although recombinant/ascites-derived antibodies are typically non-infectious, treat as potentially biohazardous per institutional policy.
Incompatibilities: Avoid strong acids/bases, oxidants, and proteases. Do not autoclave.
Preservatives: If present (e.g., sodium azide), observe specific hazards (azide is toxic; avoid copper/lead plumbing). Note: Presence/concentration not specified for this item; consult SDS/label.
First aid (overview; defer to SDS): In case of skin/eye contact, rinse with water. If inhaled, move to fresh air. If ingested, rinse mouth and seek medical advice. Provide SDS to healthcare personnel.
Spill response: Absorb with inert material, wash area with detergent solution. Dispose per local regulations.
Solvent Selection
Applicability
As a protein reagent, this antibody is intended for use in aqueous buffers rather than organic solvents.
General buffer compatibility (literature/general guidance)
Working buffers: PBS or TBS at pH ~7.2–7.6 are commonly used for dilution and washing.
Additives: 0.05–0.1% Tween-20 may reduce nonspecific binding in immunoassays. Carrier protein (e.g., 0.1–1% BSA) can improve stability and reduce adsorption; presence in the supplied material is not specified for this item.
Avoid: High concentrations of denaturants (urea, guanidine), strong detergents (SDS for native assays), and organic solvent >10–20% v/v, which can destabilize IgG.
pH and ionic strength
Most IgGs are stable close to neutral pH; extreme pH can denature or elute bound antibodies from antigens (used intentionally in affinity purification, not routine handling).
When to choose alternative diluents
For live-cell applications or azide-sensitive systems (e.g., HRP), use azide-free buffers. For protein A/G binding or purification workflows, maintain physiological salt to preserve Fc interactions.
Storage and Reconstitution
Item-specific (from Product Data)
Storage: Store at −20 °C. Avoid repeated freezing and thawing.
Shipping: Ice chest + ice pads.
General guidance (literature/best practice; not product-specific requirements)
Upon receipt: Inspect for container integrity. If frozen, thaw on ice. Mix gently by inversion; avoid vigorous vortexing.
Aliquoting: Immediately aliquot into single-use portions to minimize freeze–thaw cycles. Use low-protein-binding tubes.
Long-term storage: Many antibodies remain stable at −80 °C for extended periods; the optimal temperature and stability for this product are not specified — validate for your workflow.
Working solutions: Prepare in PBS or TBS (pH ~7.4) with optional carrier protein (e.g., 0.1–1% BSA) to reduce surface adsorption. Preservatives such as 0.02–0.05% sodium azide are commonly used for storage at 2–8 °C; compatibility for this item is not specified.
Freeze–thaw: Avoid repeated cycles; each thaw can increase aggregation and reduce activity. Keep on ice during setup.
Sterility: If aseptic handling is required, filter through a 0.22 µm membrane; assess impact on concentration/recovery.
Documentation: Record aliquot map, dates, and usage to support reproducibility and troubleshooting.
Structure and Identity
Item-specific (from Product Data)
Product: DNAJB5 Mouse mAb (monoclonal antibody, mouse origin)
SKU: Ab100149
Category: Antibody (research use only)
CAS, CID, InChIKey, SMILES: Not applicable to antibodies; not specified for this item; refer to CoA/Spec Sheet.
Literature/general description (for context; not item-specific specs)
Target: DNAJB5 (DnaJ/Hsp40 family member B5), a J-domain co-chaperone of Hsp70.
Antibody class: Typical mouse monoclonal antibodies are IgG isotypes with two heavy and two light chains forming a Y-shaped glycoprotein (~150 kDa total). Exact isotype/sequence for this product is not specified.
Epitope: Not specified for this item; refer to CoA/Spec Sheet.
2D/structural features (general for IgG)
Two antigen-binding fragments (Fab) with variable regions confer specificity to DNAJB5; one Fc region mediates effector functions in immunoassays. Disulfide bonds link heavy–heavy and heavy–light chains.
Glycosylation on Fc is typical for IgG; pattern is clone/production dependent (not specified for this item).
Synthetic Utility
Not applicable
As a mouse monoclonal antibody specific for DNAJB5, this product is not a synthetic building block or reagent and has no direct role in chemical synthesis. See Reaction & Applications for relevant biological assay uses.
Target Specificity
Item-specific (from Product Name)
Antigen/Target: DNAJB5 (DnaJ/Hsp40 family member B5)
Antibody type: Mouse monoclonal antibody (mAb)
Not specified for this item; refer to CoA/Spec Sheet
Clone designation
Isotype/subclass (e.g., IgG1, IgG2a, etc.)
Species reactivity (human/mouse/rat, etc.)
Epitope mapping/peptide immunogen
Cross-reactivity data and blocking peptide availability
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