등급 & 순도Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
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보관 및 배송
Store at -20°C,Avoid repeated freezing and thawing Ice chest + Ice pads 출고. 정확한 사양은 로트별 COA를 확인하세요.
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품질 문서
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
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문헌적 근거
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
개요
Rabbit anti Human DOK7 Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.
사양
Product Name
DOK7 Antibody - 1차 항체, specific to DOK7, Rabbit IgG
동의어
DOK 7 antibody | Docking protein 7 antibody | DOK7 antibody | DOK7_HUMAN antibody | Downstream of tyrosine kinase 7 antibody | Protein Dok-7 antibody | CMS10 | CMS1B | FADS3 | C4orf25
사양 및 순도
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
숙주 종
토끼
특이성
DOK7
면역원
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DOK7.
포지티브 제어
WB: Mouse brain, Rat brain lysates. IHC: Human brain tissue. ICC/IF: Hep G2 cells.
변화
Unconjugated
등급
Carrier Free, ExactAb™, Validated
아이소타입
Rabbit IgG
SDS-PAGE
150 kDa
정화 방법
Immunogen affinity purified
집중력
1.0 mg/mL
Product Line
생물 제품
보관 및 배송
모양
Liquid
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
DOK7 Antibody (Ab100303) - Western Blot All lanes: DOK7 Antibody (Ab100303) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/10000 dilution
Predicted band size: 53 kDa Observed band size: 56 kDa Exposure time: 32.0 s
DOK7 Antibody (Ab100303) - IF/ICC Immunofluorescent analysis of DOK7 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with DOK7 Antibody (Ab100303) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DOK7 Antibody (Ab100303) - IHC Immunohistochemical analysis of DOK7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with DOK7 Antibody (Ab100303) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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