GRADE & PURITYMoligand™?Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools.
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Application Protocols
No tested application protocols are provided for this item.
General HTS/assay handling practices (non item-specific):
Prepare a concentrated DMSO stock (e.g., 10–50 mM) in a low-bind vial; vortex and, if needed, sonicate briefly to aid dissolution.
Filter stocks through 0.2 µm PTFE if particle-free solutions are required for optical assays.
Dilute into assay buffer to the desired concentration maintaining low final DMSO (≤0.1–1% v/v as assay permits). Include vehicle controls.
For enzyme assays, pre-incubate compound with enzyme where mechanism suggests time-dependent inhibition; run appropriate controls for aggregation (detergent controls; dynamic light scattering if available).
Follow your lab’s SOPs and consult assay-specific literature for parameterization. These are general recommendations and not item-specific instructions.
DX-9065a is widely cited in the literature as a small-molecule, direct inhibitor used to interrogate the coagulation cascade by modulating the activity of a serine protease in the common pathway (often referenced as factor Xa). It has served as a benchmark tool compound for enzymology and anticoagulation mechanism studies in vitro.
As a research tool, such inhibitors enable:
Target validation via enzyme kinetics (IC50/Ki determination), selectivity panels across related serine proteases, and mechanistic studies (competitive vs. non-competitive profiles).
Pathway mapping in reconstituted systems using chromogenic or fluorogenic substrates to quantify rate changes upon inhibitor addition.
Structure–activity relationship (SAR) comparisons when combined with analog sets.
Important constraints:
This product is designated For research use only and is not intended for diagnostic or therapeutic use.
Biological potency, selectivity, and off-target profiles are assay- and lot-dependent; verify under your experimental conditions.
Item-specific notes:
No potency, selectivity, or formulation data are provided in this listing. Consult primary literature and your lot’s CoA for any available characterization.
Buffer Applications
This item is a discrete small-molecule screening compound and is not itself a buffering agent.
For biochemical assays using this compound, prepare appropriate assay buffers (e.g., HEPES, Tris, or PBS) suited to your target enzyme and detection chemistry. Keep final DMSO percentage low to minimize effects on enzyme kinetics.
Optimize ionic strength, pH, and cofactors for your target system independently of the compound.
No buffer recipes or pH specifications are provided for this product.
Green Alternatives
Green solvent/reagent substitution is not typically applicable to this listing because DX-9065a is a discrete screening compound rather than a process solvent or common reagent.
For greener practices in handling small-molecule libraries:
Favor aqueous assay buffers with minimal DMSO where feasible.
Use microscale formats and sealed plates to reduce solvent consumption and emissions.
Implement solvent recycling for washing steps (e.g., ethanol) where validated.
No direct “alternative compound” fulfills the same bioactivity profile; selection should be based on your biological target rather than green chemistry criteria.
Pharmaceutical Uses
No pharmacopeial or excipient role is specified for this product. It is supplied as a research-use screening compound.
Research-use note: Not intended for human or veterinary use, drug substance manufacturing, or clinical applications.
If employing as a reference standard in analytical method development, qualify the material internally for identity and purity relative to your method requirements.
Item-specific regulatory or formulation details are not provided; refer to the CoA/Spec Sheet and institution SOPs.
Physical Properties
Item-specific physico-chemical specifications have not been provided for this catalog entry.
Appearance (item-specific): Not specified for this item; refer to CoA/Spec Sheet.
Melting point / boiling point (item-specific): Not specified for this item; refer to CoA/Spec Sheet.
Density / refractive index (item-specific): Not specified for this item; refer to CoA/Spec Sheet.
Solubility (item-specific): Not specified for this item; refer to CoA/Spec Sheet.
LogP, pKa (item-specific): Not specified for this item; refer to CoA/Spec Sheet.
General guidance (literature/practice, not item-specific):
Screening compounds of this class are commonly handled as DMSO stock solutions (e.g., 10–50 mM) for assay use due to broad solubility in polar aprotic media.
Aqueous solubility of hydrophobic enzyme inhibitors can be low; use co-solvents (DMSO) and surfactant-free buffers to avoid colloidal aggregation in bioassays.
Always verify the actual salt form; counter-ions can significantly alter aqueous solubility and pH-dependent behavior.
Quality and Grades
Supplied grade (item-specific): Moligand™.
Moligand™ designates Aladdin’s small-molecule library/ligand screening grade intended for discovery research, assay development, and target validation.
Typical attributes of library-grade materials include identity confirmation (e.g., MS/NMR), chromatographic purity suitable for screening, and packaging amenable to plate-based workflows. Exact acceptance criteria for this specific item are not provided here.
Purity/assay (item-specific): Not specified for this item; refer to CoA/Spec Sheet for analytical data (e.g., HPLC area %, residual solvents, water content) corresponding to your lot.
Stabilizers/inhibitors (item-specific): Not specified for this item; refer to CoA/Spec Sheet.
What this means in practice:
Appropriate for biochemical/biophysical assays, cell-free target studies, and early cellular screening where library-grade purity is acceptable.
For quantitative SAR or advanced lead optimization, users may require re-purification and expanded characterization (chiral purity, salt form verification, water content), depending on assay sensitivity.
Documentation: Each lot ships with or has available a Certificate of Analysis (CoA) detailing the analytical methods and results. Use the CoA/Spec Sheet as the authoritative source for specifications.
Regulatory status: For research use only. Not intended for diagnostic, therapeutic, or clinical applications.
Reaction and Applications
This product is supplied as a bioactive small-molecule standard for research screening rather than as a synthetic reagent.
Not typically used as a solvent, reagent, or catalyst in preparative chemistry.
If employing the compound for immobilization or probe development, standard bioconjugation or linker-installation chemistries must be tailored to the actual functional groups present in your lot’s structure; confirm by spectroscopic analysis before route design.
No manufacturer reaction-use applications have been provided for this item.
Reaction Conditions
Not applicable as a general reagent. No synthetic reaction condition guidance is provided for this item.
For any custom modifications, standard organic synthesis conditions (solvent, base/acid, temperature, catalyst) should be selected based on the actual functional groups present once confirmed by analytical characterization.
No manufacturer-recommended reaction conditions are available.
Safety and Handling
Safety details specific to this item have not been supplied; consult the SDS provided with your shipment for authoritative guidance.
GHS classification (item-specific): Not specified for this item; refer to SDS.
Signal word / H-statements / pictograms (item-specific): Not specified for this item; refer to SDS.
General laboratory precautions (good practice):
PPE: Lab coat, safety glasses, disposable nitrile gloves. Handle powders/solids in a fume hood to avoid inhalation of dust or aerosols.
Avoid exposure: Prevent skin/eye contact and ingestion. Wash thoroughly after handling. Do not pipette by mouth.
Incompatibilities: Avoid strong oxidizers and strong acids/bases until compatibility is known (general precaution for organic small molecules).
Hygiene: Use dedicated tools for weighing to prevent cross-contamination across screening libraries.
First aid (overview): In case of contact, rinse skin or eyes with water for several minutes. If inhaled, move to fresh air. If ingested, rinse mouth and seek medical advice. Always follow your institution’s EHS procedures.
Waste: Collect as halogenated or non-halogenated organic waste according to local regulations and the compound’s SDS guidance.
Transport/storage stability: Keep container tightly closed; protect from direct sunlight and moisture. Use secondary containment to prevent spills of DMSO stock vials.
Solvent Selection
As a screening library small molecule, DX-9065a is typically handled in organic stock solutions for compatibility with biological assays.
Primary stock solvent (general practice): DMSO is commonly used due to broad solubility and compatibility with high-throughput screening (HTS) workflows.
Assay dilution: Dilute DMSO stocks into aqueous buffers immediately before use; maintain final DMSO typically ≤0.1–1% v/v to minimize assay interference (assay-dependent).
Potential co-solvents (general): DMF, ethanol, or aqueous buffers containing minimal co-solvent. Confirm actual solubility and stability for your lot.
pH considerations: Solubility and ionization can be pH-dependent for heteroatom-rich scaffolds; evaluate performance across assay pH if required.
Comparison (general):
DMSO: highest solvating power; widely compatible with HTS; can influence enzyme kinetics at higher percentages.
Ethanol: more volatile; lower solvating power; sometimes preferable for rapid evaporation steps.
Aqueous buffers: best for biological relevance; may require co-solvent or cyclodextrins if intrinsic solubility is low.
Item-specific properties (solubility, pKa, logP) are not provided for this product; consult the CoA/Spec Sheet and perform a quick solubility screen in your intended assay media.
Shipped in: Not specified for this item; refer to shipping documents.
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Item-specific reconstitution data are not provided.
General guidance (not item-specific; verify with your lot):
Upon receipt, store tightly closed in a desiccated, light-protected environment at room temperature unless your CoA indicates otherwise.
For working solutions, dissolve in anhydrous DMSO to prepare a concentrated stock (e.g., 10–50 mM). Record exact concentration by weight and volume.
Aliquot stocks into small, single-use vials or plate wells to minimize freeze–thaw and headspace moisture exposure.
Short-term storage of DMSO stocks: 2–8°C or room temperature in the dark; longer-term: −20°C or −80°C as compatible with your lab practice. Avoid repeated freeze–thaw cycles.
Before assay, allow frozen stocks to equilibrate to room temperature to prevent water condensation; mix thoroughly and inspect for precipitation.
For definitive, lot-specific instructions, consult the CoA/Spec Sheet and SDS provided with the product.
Structure and Identity
Brief overview: DX-9065a is supplied as a Moligand-grade small-molecule library member intended for research screening and assay development.
Product identifiers (item-specific):
SKU: D610027 (Aladdin Scientific)
Product name: DX-9065a
CAS: 155204-81-2
Category: Small molecules and compound libraries (screening compound)
Structure descriptors (item-specific):
Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
Structural features (general/literature context, not item-specific):
DX-9065a is reported in the literature as a synthetic, non-peptidic small molecule developed as a selective enzyme inhibitor (see Biological Roles) used widely as a reference tool compound in coagulation biology studies.
Exact 2D connectivity, salt/solvate form, and stereochemical specification may vary by supplier or lot; confirm using the CoA for the specific batch you receive.
Practical note: For definitive identity confirmation in your lab, use orthogonal techniques (e.g., HRMS, 1H/13C NMR, and HPLC/UPLC retention time) against an authenticated reference if your workflow requires structural verification.
Synthetic Utility
This product is not offered as a building block or reagent; synthetic utility is not a primary use case.
If derivatization is contemplated for probe development (e.g., affinity tags, photo-crosslinkers), confirm the exact functional group map of your lot (by NMR/MS). Choice of coupling chemistry (amide formation, click reactions, carbamate/sulfonamide formation) must be guided by the confirmed structure.
Protect bioactivity during derivatization by avoiding modifications to known pharmacophores; consult literature SAR for guidance.
No item-specific functional group specifications are provided in this listing.
Target Specificity
Item-specific target data are not provided in this listing.
No antigen/epitope, enzyme target, or selectivity panel is specified by the manufacturer for this product entry.
For research planning, consult primary literature on DX-9065a to understand reported biochemical targets and selectivity, then validate in your own assays.
All biological use is for research only.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
