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Coomassie Blue Solution Recipe

Protocols

Coomassie Blue Solution Recipe

· Reviewed · Published Jun 9, 2025 · Updated Jul 27, 2023 · 8 min read

Recipe

1.Dissolve 2g Coomassie Blue (Serva Blau) in 250ml water

2.Slowly add 75ml of glacial acetic acid

3.Add 500ml of ethanol

4.q.s. to 1000ml with water

Final concentrations:

0.2% Coomassie Blue

7.5% Acetic Acid

50% Ethanol

Categories: Protocols

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Coomassie Blue Solution Recipe" Aladdin Knowledge Base, updated Jul 27, 2023. https://www.aladdinsci.com/us_en/faqs/coomassie-blue-solution-recipe-en.html

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