Recombinant Human B3GALT2 Protein: Key Facts & FAQs

Recombinant Human B3GALT2 Protein is a recombinant form of the human beta-1,3-galactosyltransferase 2 enzyme, produced for use in glycobiology research, glycan biosynthesis studies, and enzyme activity assays.

Product Name

Recombinant Human B3GALT2 Protein

SKU

rp371512

Protein Family

Beta-1,3-galactosyltransferase family (B3GALT)

Biological Function

Catalyzes transfer of galactose from UDP-galactose to acceptor substrates in beta-1,3 linkage, involved in glycan biosynthesis

Research Applications

Glycobiology research, glycan biosynthesis studies, enzyme kinetics, substrate specificity assays

Supplier

Aladdin Scientific

What Is Recombinant Human B3GALT2 Protein?

Recombinant Human B3GALT2 Protein is a recombinant version of human beta-1,3-galactosyltransferase 2, a member of the B3GALT enzyme family. B3GALT2 belongs to the glycosyltransferase superfamily and catalyzes the transfer of galactose from the nucleotide sugar donor UDP-galactose to specific acceptor oligosaccharides via a beta-1,3 glycosidic linkage. This enzyme plays a role in the biosynthesis of complex glycan structures found on glycoproteins and glycolipids, contributing to cell-surface carbohydrate diversity.

The recombinant protein is produced using heterologous expression systems, allowing researchers to obtain purified, functional enzyme suitable for in vitro experimentation. As a research reagent, Recombinant Human B3GALT2 Protein (SKU: rp371512) is supplied by Aladdin Scientific to support glycobiology and related biochemical investigations.

Research Applications of B3GALT2 Protein

Recombinant Human B3GALT2 Protein is used across a range of glycobiology and biochemical research applications, including:

• Glycan biosynthesis studies: Investigating the enzymatic assembly of beta-1,3-linked galactose-containing oligosaccharide chains on glycoproteins and glycolipids.
• Enzyme kinetics and activity assays: Characterizing catalytic parameters such as substrate specificity, donor/acceptor preferences, and turnover rates using radiometric or fluorescence-based methods.
• Substrate specificity profiling: Determining which oligosaccharide acceptors are recognized by B3GALT2, aiding understanding of its role in glycan diversity.
• Disease-related glycobiology: B3GALT family members have been implicated in cellular processes relevant to cancer biology, neurological function, and immune regulation; recombinant B3GALT2 supports mechanistic studies in these contexts.
• Drug discovery support: Evaluating small-molecule inhibitors or activators targeting glycosyltransferases.

Researchers should confirm activity specifications and suitable assay conditions with Aladdin Scientific technical support prior to experimental use.

Handling, Storage, and Stability

Proper handling and storage of Recombinant Human B3GALT2 Protein are essential to preserve enzymatic activity and protein integrity. The following general best-practice guidelines apply to recombinant proteins of this class:

• Storage temperature: Recombinant proteins are generally stored at -20°C or -80°C upon receipt. Long-term storage at -80°C is typically recommended to maximize stability.
• Avoid freeze-thaw cycles: Repeated freezing and thawing can lead to protein denaturation and loss of activity. Aliquot the protein into single-use volumes before freezing.
• Buffer and formulation: The supplied formulation buffer (which may contain stabilizing agents such as BSA or glycerol) should not be altered without validation.
• Working concentrations: Dilute immediately before use in appropriate assay buffer. Avoid prolonged incubation at room temperature.

Note: Specific storage conditions, formulation details, and shelf-life information for SKU rp371512 should be confirmed from the official product datasheet provided by Aladdin Scientific, as these parameters are product-lot specific.

Quality, Purity, and Specification Considerations

When evaluating recombinant enzyme reagents such as B3GALT2, researchers and procurement managers should review key quality attributes:

• Purity: Recombinant proteins intended for biochemical assays are typically characterized by SDS-PAGE to confirm purity level (commonly ≥85% or ≥95% depending on grade). The exact purity specification for rp371512 should be verified on the product datasheet.
• Activity validation: Functional enzyme lots may be tested using standard glycosyltransferase activity assays to confirm catalytic competency prior to release.
• Endotoxin levels: For cell-based assays or applications sensitive to endotoxin contamination, the endotoxin content (typically measured in EU/mg) should be reviewed in the certificate of analysis (CoA).
• Host expression system: The expression host (e.g., E. coli, insect cells, HEK293 mammalian cells) affects post-translational modifications. Mammalian or insect cell expression is generally preferred for glycosyltransferases to retain proper folding and, where applicable, glycosylation.

Request the certificate of analysis and product datasheet for lot-specific quality data when ordering SKU rp371512 from Aladdin Scientific.

Ordering, Support, and Technical Assistance

Recombinant Human B3GALT2 Protein (SKU: rp371512) is available for order directly through Aladdin Scientific. When placing an order or seeking technical guidance, the following resources are available:

• Product page: Visit the Aladdin Scientific website and search SKU rp371512 for up-to-date pricing, availability, and datasheet downloads.
• Technical support: Contact Aladdin Scientific technical support for guidance on experimental design, optimal assay conditions, and compatibility with downstream applications.
• Certificate of Analysis (CoA): Available upon request or downloadable by lot number from the Aladdin Scientific website; contains lot-specific purity, activity, and endotoxin data.
• Bulk and custom orders: For large-volume requirements or custom formulations, contact the Aladdin Scientific sales team directly.
• Returns and refunds: Refer to Aladdin Scientific's official returns and refunds policy for eligibility criteria applicable to recombinant protein products.

Frequently asked questions

What is the biological function of human B3GALT2?

B3GALT2 (beta-1,3-galactosyltransferase 2) is an enzyme that catalyzes the transfer of galactose from UDP-galactose to oligosaccharide acceptors via a beta-1,3 glycosidic linkage. It is involved in the biosynthesis of complex glycan structures on glycoproteins and glycolipids, contributing to cell-surface carbohydrate diversity and associated biological recognition events.

What expression system is used to produce Recombinant Human B3GALT2 Protein (rp371512)?

The specific expression host used for rp371512 should be confirmed by consulting the official Aladdin Scientific product datasheet or certificate of analysis, as expression system details are product- and lot-specific. Common systems for recombinant glycosyltransferases include E. coli, insect cells (baculovirus), and mammalian cell lines such as HEK293, with mammalian or insect systems preferred when native folding or glycosylation is important.

How should Recombinant Human B3GALT2 Protein be stored to maintain activity?

As a general best practice for recombinant proteins, B3GALT2 should be stored at -80°C for long-term preservation and aliquoted into single-use volumes to avoid repeated freeze-thaw cycles, which can cause protein denaturation and loss of enzymatic activity. The exact recommended storage conditions for rp371512 are specified in the Aladdin Scientific product datasheet and should be followed strictly.

What quality documentation is available for rp371512?

Aladdin Scientific provides a Certificate of Analysis (CoA) for each lot of rp371512, which includes lot-specific data on purity, activity, and endotoxin levels. The product datasheet containing general specifications and recommended usage conditions is also available. These documents can be requested from Aladdin Scientific customer support or downloaded via the product page on the Aladdin Scientific website.

Is Recombinant Human B3GALT2 Protein suitable for cell-based assays?

Suitability for cell-based assays depends on the endotoxin content and formulation of the specific lot. Researchers planning to use rp371512 in cell-based applications should review the CoA for endotoxin levels (measured in EU/mg) and confirm compatibility with their cell system. Contact Aladdin Scientific technical support for specific guidance on cell-based application compatibility.

References

1. Kolbinger F, Streiff MB, Katopodis AG. Cloning of a human UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase catalyzing the formation of type 1 chains. Journal of Biological Chemistry. 1998;273(1):433-440.
2. Breton C, Snajdrová L, Jeanneau C, Koca J, Imberty A. Structures and mechanisms of glycosyltransferases. Glycobiology. 2006;16(2):29R-37R.
3. Aladdin Scientific. Recombinant Human B3GALT2 Protein, SKU rp371512. Product datasheet. Aladdin Scientific; [date unspecified].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.