Recombinant Human C7orf25 Protein: Properties & Research Use

Recombinant Human C7orf25 Protein is a research-grade recombinant protein derived from the human chromosome 7 open reading frame 25 gene, produced for use in biochemical assays, protein interaction studies, and functional research applications.

Product Name

Recombinant Human C7orf25 Protein

SKU

rp376475

Gene

C7orf25 (Chromosome 7 Open Reading Frame 25)

Species Origin

Human (Homo sapiens)

Protein Type

Recombinant protein

Recommended Use

Biochemical assays, protein interaction studies, functional research

What Is C7orf25 and Why Study It?

C7orf25, also known as Chromosome 7 Open Reading Frame 25, is a human protein-coding gene located on chromosome 7. While the precise biological function of C7orf25 remains an active area of investigation, open reading frame (ORF) proteins encoded on chromosome 7 are of broad interest due to the chromosome's association with multiple developmental and oncological processes.

Recombinant versions of such proteins enable researchers to investigate protein structure, potential binding partners, enzymatic activity, and regulatory roles in a controlled in vitro environment. The availability of a high-purity recombinant form of C7orf25 supports hypothesis-driven research into its cellular function and potential disease relevance.

Protein Production and Quality Attributes

Recombinant Human C7orf25 Protein (SKU: rp376475) is produced using established recombinant protein expression systems. Recombinant proteins of this class are typically expressed in bacterial, insect, or mammalian cell systems, followed by affinity chromatography purification to achieve research-grade purity.

Quality attributes relevant to recombinant proteins include assessed purity (commonly evaluated by SDS-PAGE), endotoxin levels, and biological activity confirmation where applicable. Researchers should consult the product datasheet for the specific lot-level quality control data associated with SKU rp376475.

Note: Specific expression host, purity percentage, and endotoxin specifications for this product should be verified against the current product datasheet from Aladdin Scientific.

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins are critical to maintaining integrity and activity over time. As a general best practice for recombinant proteins:

• Store lyophilized protein at –20 °C or –80 °C as specified in the datasheet, protected from moisture.
• Reconstitute lyophilized material in the recommended buffer (e.g., sterile PBS or supplier-specified diluent) and mix gently; avoid vigorous vortexing.
• Aliquot reconstituted protein to minimize freeze-thaw cycles, which can cause protein aggregation and loss of activity.
• For short-term use (up to 1 week), store reconstituted protein at 4 °C; for longer storage, freeze aliquots at –80 °C.

Always refer to the current Aladdin Scientific product datasheet for product-specific storage conditions and shelf life.

Research Applications

Recombinant Human C7orf25 Protein is suitable for a range of in vitro research applications. Typical uses for recombinant ORF proteins include:

• Protein–protein interaction assays: Use as bait or prey in pull-down, co-immunoprecipitation, or yeast two-hybrid validation experiments to identify binding partners.
• Antibody development and validation: Serve as an antigen standard for generating or validating anti-C7orf25 antibodies.
• Biochemical characterization: Investigate potential enzymatic or structural functions through in vitro assays.
• Cell-based studies: Introduce exogenous recombinant protein into cell culture models to study functional outcomes.
• Western blot positive control: Use as a molecular weight and immunoreactivity reference in immunoblotting experiments.

Researchers are encouraged to optimize assay conditions, including protein concentration and buffer compatibility, for their specific experimental systems.

Ordering and Technical Support

Recombinant Human C7orf25 Protein is available from Aladdin Scientific under SKU rp376475. To place an order or request a formal quotation, contact your regional Aladdin Scientific representative or visit the product page on the Aladdin Scientific website.

For technical questions regarding reconstitution protocols, assay optimization, or product-specific quality data, Aladdin Scientific's technical support team is available to assist. Please have your SKU and lot number ready when contacting support to ensure prompt and accurate assistance.

Bulk quantities and custom formulations may be available upon request; inquire with the sales team for pricing and lead-time information.

Frequently asked questions

What is Recombinant Human C7orf25 Protein used for?

Recombinant Human C7orf25 Protein is used in a variety of in vitro research applications, including protein–protein interaction studies, antibody development and validation, biochemical characterization, cell-based functional assays, and as a positive control in Western blotting experiments.

How should Recombinant Human C7orf25 Protein be stored?

As a general guideline, lyophilized recombinant proteins should be stored at –20 °C or –80 °C in a dry environment. After reconstitution, aliquot the protein and store aliquots at –80 °C to avoid repeated freeze-thaw cycles. Always consult the Aladdin Scientific product datasheet for product-specific storage instructions.

What is the SKU for Recombinant Human C7orf25 Protein from Aladdin Scientific?

The SKU for Recombinant Human C7orf25 Protein available from Aladdin Scientific is rp376475. Please reference this SKU when placing orders or contacting technical support.

Is the biological function of C7orf25 fully characterized?

The precise biological function of C7orf25 is not yet fully characterized and remains an active area of scientific investigation. Recombinant C7orf25 protein provides researchers with the tool needed to study its potential roles in protein interactions, cellular processes, and disease-relevant pathways.

References

1. UniProt Consortium. UniProt: the universal protein knowledgebase. Nucleic Acids Research. Entry for C7orf25 (Human).
2. NCBI Gene. C7orf25 chromosome 7 open reading frame 25 [Homo sapiens]. National Center for Biotechnology Information.
3. Aladdin Scientific. Recombinant Human C7orf25 Protein, SKU rp376475. Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.