Recombinant Human CEP126 Protein: Research Guide

Recombinant Human CEP126 Protein is a centriolar satellite-associated protein reagent designed to support mechanistic studies of ciliogenesis, centrosome biology, and related ciliopathy research.

Product Name

Recombinant Human CEP126 Protein

SKU

rp374590

Protein Origin

Human (Homo sapiens)

Protein Class

Centriolar satellite / centrosome-associated protein

Primary Research Application

Ciliogenesis, centrosome biology, ciliopathy studies

Format

Recombinant protein; specific formulation details should be confirmed on the product datasheet

Supplier

Aladdin Scientific

Background: CEP126 and Its Role in Ciliogenesis

CEP126 (Centrosomal Protein 126) is a component associated with centriolar satellites—electron-dense granules that cluster around the centrosome and basal body in mammalian cells. Centriolar satellites play essential roles in the trafficking of proteins to and from the centrosome, and are critically involved in the initiation and regulation of ciliogenesis, the cellular process by which primary cilia are assembled.

CEP126 has been identified as a cilia-related protein whose expression and localization are tightly coupled to the cell cycle. Studies indicate that CEP126 localizes to centriolar satellites and contributes to the structural and regulatory framework necessary for proper cilium formation. Disruption of CEP126 function has been linked to defects in ciliogenesis, placing it within the broader family of proteins relevant to ciliopathies—a spectrum of human diseases caused by dysfunctional cilia, including nephronophthisis, Bardet-Biedl syndrome, and Joubert syndrome.

As a recombinant human protein, this reagent enables researchers to perform in vitro biochemical assays, interaction studies, and functional reconstitution experiments without dependence on endogenous protein sources, providing greater experimental control and reproducibility.

Protein Properties and Formulation Considerations

Recombinant Human CEP126 Protein (rp374590) is produced as a recombinant form of the human CEP126 gene product. Researchers should consult the current product datasheet available from Aladdin Scientific for confirmed specifications including molecular weight, expression system (e.g., mammalian, insect, or E. coli), tag configuration (such as His-tag or GST-tag), purity grade, and buffer composition, as these parameters are critical for downstream experimental design.

General considerations applicable to recombinant proteins of this class include:

• Purity: Typical recombinant proteins intended for research use are supplied at ≥85–95% purity as assessed by SDS-PAGE; confirm the exact specification on the datasheet.
• Endotoxin levels: For cell-based assays, endotoxin content should be verified; the datasheet will specify the tested endotoxin level.
• Reconstitution: Lyophilized preparations should be reconstituted according to the datasheet instructions, typically in sterile deionized water or an appropriate buffer, with gentle mixing to avoid protein denaturation.
• Carrier proteins: Some formulations include BSA as a stabilizer; verify compatibility with your assay system before use.

Storage, Handling, and Stability Guidelines

Proper storage and handling are essential to maintain the biological activity and structural integrity of Recombinant Human CEP126 Protein. Adhere to the following best-practice guidelines unless otherwise specified in the product datasheet:

• Long-term storage: Store lyophilized protein at –20 °C or –80 °C, protected from moisture and light. Liquid formulations should be stored at the temperature specified on the datasheet, typically –80 °C for extended storage.
• Aliquoting: Upon reconstitution, prepare single-use aliquots immediately to minimize freeze-thaw cycles, which can reduce protein activity and promote aggregation.
• Freeze-thaw cycles: Avoid repeated freeze-thaw cycles; it is recommended to limit cycles to no more than 2–3 times. Each cycle can lead to partial denaturation and loss of functional activity.
• Working concentration: Determine the optimal working concentration empirically for your specific assay. Pilot titration experiments are recommended before committing to large-scale assays.
• Contamination prevention: Use sterile technique when handling the protein. Avoid introducing proteases, detergents, or other denaturing agents into the stock solution.

Always refer to the specific storage conditions printed on the product label and detailed in the current datasheet, as formulation-specific requirements take precedence over general guidance.

Experimental Applications

Recombinant Human CEP126 Protein is suitable for a range of in vitro and cell-free research applications. Representative applications include:

• Protein–protein interaction studies: CEP126 can be used as bait or prey in pull-down assays, co-immunoprecipitation validation experiments, or surface plasmon resonance (SPR) binding studies to map its interactome within the centriolar satellite complex.
• Biochemical characterization: The recombinant protein supports enzymatic or structural studies, including circular dichroism (CD) spectroscopy for secondary structure analysis and thermal shift assays to assess stability.
• Western blot standard: Recombinant CEP126 can serve as a positive control or molecular weight standard in Western blot experiments using anti-CEP126 antibodies.
• ELISA standard curve generation: The purified protein can be used to generate standard curves for quantitative sandwich ELISA assays measuring endogenous CEP126 levels in biological samples.
• Functional reconstitution: In cell-free systems, the recombinant protein may be used to study the reconstitution of centriolar satellite assembly or to assess the impact of post-translational modifications on CEP126 interactions.
• Antibody validation: The protein is suitable for validating the specificity of anti-CEP126 antibodies through dot blot or immunoprecipitation experiments.

Researchers should validate the protein's activity in each specific assay format, as performance may vary depending on assay conditions and the expression system used to produce the recombinant protein.

Frequently asked questions

What is CEP126 and why is it important in cilia research?

CEP126 is a centriolar satellite-associated protein that plays a key role in regulating ciliogenesis—the formation of primary cilia—in mammalian cells. It contributes to the structural organization of centriolar satellites and the trafficking of ciliary components to the basal body. Disruption of CEP126 is associated with defects in cilia assembly, linking it to ciliopathy research relevant to diseases such as nephronophthisis and Joubert syndrome.

How should I store Recombinant Human CEP126 Protein to preserve its activity?

For long-term storage, lyophilized Recombinant Human CEP126 Protein should be kept at –20 °C or –80 °C in a dry environment, protected from light. After reconstitution, aliquot the protein immediately and avoid repeated freeze-thaw cycles—limit to 2–3 cycles maximum. Always refer to the specific storage instructions on the Aladdin Scientific product datasheet (SKU: rp374590), as formulation details take precedence.

What expression system is used to produce Recombinant Human CEP126 Protein?

The specific expression system used for Recombinant Human CEP126 Protein (rp374590) should be confirmed by consulting the official product datasheet from Aladdin Scientific, as this detail is critical for interpreting experimental results, particularly with regard to post-translational modifications and protein folding. Common systems for recombinant proteins include E. coli, insect cells, and mammalian expression systems.

Can Recombinant Human CEP126 Protein be used as a positive control in Western blotting?

Yes, Recombinant Human CEP126 Protein (rp374590) can serve as a positive control or molecular weight reference in Western blot experiments using anti-CEP126 antibodies. It is also suitable for validating antibody specificity in dot blot and immunoprecipitation assays. Verify compatibility with your specific antibody clone and detection system before use.

What quality controls are performed on Recombinant Human CEP126 Protein?

Quality control specifications—including purity assessment by SDS-PAGE, endotoxin testing, and functional activity validation—are detailed in the product datasheet for rp374590. Researchers should download the current datasheet from Aladdin Scientific's website or contact technical support for the most up-to-date QC data.

References

1. Wilkinson CJ et al. A SUMO-dependent protein interaction network regulates the formation of centriolar satellites. EMBO Rep. 2014; referenced as context for centriolar satellite biology.
2. Lopes CA et al. Centriolar satellites are assembly points for proteins implicated in human ciliopathies, including oral-facial-digital syndrome 1. J Cell Sci. 2011;124(Pt 4):600-612.
3. Braun DA, Hildebrandt F. Ciliopathies. Cold Spring Harb Perspect Biol. 2017;9(3):a028191. doi:10.1101/cshperspect.a028191

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.