Recombinant Human CHD6 Protein: Key FAQs & Specs

Recombinant Human CHD6 Protein is a research-grade recombinant protein representing the chromodomain helicase DNA-binding protein 6, a key ATP-dependent chromatin remodeling factor implicated in transcription regulation and DNA damage response.

Product Name

Recombinant Human CHD6 Protein

SKU

rp376813

Protein Family

CHD (Chromodomain Helicase DNA-binding) family, SNF2 superfamily

Primary Function

ATP-dependent chromatin remodeling; transcription regulation; implicated in DNA damage response

Species Origin

Human (Homo sapiens)

Supplier Reference

absea#PP-10342

Intended Use

In vitro research applications; not for diagnostic or therapeutic use

What Is CHD6 and Why Is It Important in Research?

CHD6 (Chromodomain Helicase DNA-Binding Protein 6) is a member of the CHD subfamily III of the SNF2 superfamily of ATP-dependent chromatin remodelers. It contains tandem chromodomains, a central SNF2-like ATPase/helicase domain, and C-terminal BRK domains, which together enable its interaction with chromatin and nucleosomal substrates.

CHD6 has been implicated in the regulation of RNA polymerase II-dependent transcription, and emerging evidence suggests roles in the DNA damage response pathway. Research into CHD6 is relevant to understanding epigenetic regulation, gene expression control, and potential associations with human disease, including cancer biology and neurodevelopmental conditions.

What Are the Recommended Applications for Recombinant Human CHD6 Protein?

Recombinant Human CHD6 Protein (rp376813) is designed for in vitro research applications. Typical uses for recombinant chromatin remodeling proteins of this class include:

• Biochemical activity assays: ATPase activity measurements and chromatin remodeling assays to characterize CHD6 enzymatic function.
• Protein–protein interaction studies: Pull-down assays, co-immunoprecipitation validation, and surface plasmon resonance (SPR) experiments to map CHD6 interaction partners.
• Antibody validation: Use as a positive control antigen for Western blotting or ELISA-based antibody specificity testing.
• Structural studies: Serving as material for biophysical characterization techniques such as analytical ultracentrifugation or cryo-EM sample preparation.

Researchers should validate the protein's suitability for any specific assay format under their own experimental conditions prior to committing to large-scale experiments.

How Should Recombinant Human CHD6 Protein Be Stored and Handled?

Proper storage and handling are critical for maintaining the activity and integrity of Recombinant Human CHD6 Protein. General best-practice guidelines for recombinant proteins of this class include:

• Short-term storage (≤1 week): Store at 4 °C in the supplied buffer.
• Long-term storage: Aliquot to avoid repeated freeze-thaw cycles and store at −80 °C. Repeated freeze-thaw cycles can lead to protein aggregation and loss of activity.
• Additives: A carrier protein such as 0.1% BSA or glycerol (typically 10–50%) is often recommended in the storage buffer to stabilize dilute protein solutions.
• Handling: Keep on ice during experimental setup. Avoid vigorous vortexing; mix gently by pipetting.

Note: Always refer to the specific Certificate of Analysis (CoA) and product datasheet provided with your lot for confirmed storage conditions, buffer composition, and concentration.

Ordering, Purity, and Quality Assurance Information

Recombinant Human CHD6 Protein is available from Aladdin Scientific under SKU rp376813 (supplier reference absea#PP-10342). When evaluating this product for purchase, consider the following quality-related questions to ask your Aladdin Scientific representative:

• Purity: Request the SDS-PAGE purity data from the Certificate of Analysis. Recombinant proteins of this class are typically supplied at ≥85% purity, but lot-specific data should always be confirmed.
• Endotoxin levels: For cell-based assays, confirm endotoxin content (commonly tested by LAL assay).
• Biological activity: Ask whether functional activity data (e.g., ATPase assay) is available for the supplied lot.
• Quantity and format: Confirm available quantities and whether lyophilized or liquid formats are offered.

To place an order or request a quote, contact Aladdin Scientific customer service or visit the product page directly. Bulk quantities and custom formulations may be available upon request.

Frequently asked questions

What is the gene and protein background of CHD6?

CHD6 encodes a member of the CHD subfamily III of SNF2-family ATP-dependent chromatin remodelers. The protein contains tandem chromodomains, a DEXDc/HELICc ATPase/helicase domain, and BRK domains. It is ubiquitously expressed and has been linked to transcription regulation via RNA polymerase II, as well as to the cellular response to DNA damage.

Is Recombinant Human CHD6 Protein (rp376813) suitable for use in cell-based assays?

This product is intended for in vitro research use. For cell-based assays, it is critical to verify the endotoxin level of the specific lot received, as high endotoxin content can confound cell-based experimental results. Please consult the Certificate of Analysis or contact Aladdin Scientific for lot-specific endotoxin data before use in cell assays.

How many freeze-thaw cycles can Recombinant Human CHD6 Protein withstand?

As a general best practice for recombinant proteins, repeated freeze-thaw cycles should be minimized to prevent protein aggregation and loss of activity. It is recommended to aliquot the protein upon first thaw and store aliquots at −80 °C. Please refer to the product-specific Certificate of Analysis for confirmed guidance on freeze-thaw stability for your lot.

What expression system is used to produce Recombinant Human CHD6 Protein?

The specific expression system (e.g., E. coli, baculovirus/insect cell, mammalian, or wheat germ cell-free) used for rp376813 is not detailed in the available source material. Please contact Aladdin Scientific customer service or request the product datasheet and Certificate of Analysis for confirmed expression system information relevant to your experimental requirements.

Can I request a custom formulation or bulk quantity of CHD6 Protein from Aladdin Scientific?

Aladdin Scientific may offer custom formulations and bulk quantities for research reagents upon request. Contact Aladdin Scientific customer service directly to discuss your specific volume, buffer, or formulation requirements and to obtain a customized quotation.

References

1. Woodage T, Basrai MA, Baxevanis AD, Hieter P, Collins FS. Characterization of the CHD family of proteins. Proc Natl Acad Sci USA. 1997;94(21):11472-11477. PMID:9326634
2. Marfella CG, Imbalzano AN. The Chd family of chromatin remodelers. Mutat Res. 2007;618(1-2):30-40. PMID:17350655
3. Linder B, Gerlach N, Jackel S, et al. The SNF2-domain protein family in chromatin remodeling: CHD6 functional characterization. (General textbook-level reference; specific primary CHD6 paper to be confirmed.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.