Recombinant Human GABA B Receptor 2 Protein: Key FAQs

Recombinant Human GABA B Receptor 2 Protein is a research-grade recombinant protein derived from the human GABBR2 gene, designed to support neuroscience studies investigating GABAergic inhibitory signaling, receptor–ligand interactions, and related therapeutic target research.

Product Name

Recombinant Human GABA B Receptor 2 Protein

SKU

rp367873

Gene Name

GABBR2

Species Origin

Human (Homo sapiens)

Protein Type

Recombinant receptor protein

Research Area

Neuroscience, GABAergic signaling, inhibitory neurotransmission

What Is Recombinant Human GABA B Receptor 2 Protein?

Recombinant Human GABA B Receptor 2 Protein (SKU: rp367873) is a recombinant form of the human GABA type B receptor subunit 2, encoded by the GABBR2 gene. GABA B receptors are metabotropic G protein-coupled receptors (GPCRs) that mediate slow, prolonged inhibitory neurotransmission in the central nervous system (CNS). The receptor functions as a heterodimer composed of GABA B1 and GABA B2 subunits. The GABA B2 subunit is primarily responsible for G protein coupling and downstream signaling, while GABA B1 binds the agonist GABA. This recombinant protein is produced to enable researchers to study receptor structure, function, protein–protein interactions, and drug target validation without requiring extraction from native tissue.

Recommended Applications and Use Cases

Recombinant Human GABA B Receptor 2 Protein is suitable for a range of in vitro research applications commonly employed in neuroscience and pharmacology laboratories. Typical applications include:

• Protein–protein interaction studies: Investigating the heterodimerization of GABA B1 and GABA B2 subunits or identifying novel binding partners.
• Drug discovery and target validation: Screening small molecules or biologics for GABA B receptor modulatory activity.
• Biochemical assays: Including ELISA-based binding assays, surface plasmon resonance (SPR), and pull-down experiments.
• Antibody development and validation: Serving as a positive control or coating antigen for antibody characterization.
• Structural biology: Supporting studies aimed at elucidating GABA B receptor conformation and domain interactions.

Researchers should validate the protein's suitability for each specific assay format prior to use in critical experiments.

Storage, Stability, and Handling Guidelines

Proper storage and handling are critical to maintaining the activity and integrity of recombinant proteins. General best-practice guidelines for Recombinant Human GABA B Receptor 2 Protein include:

• Storage temperature: Store at -20°C or -80°C upon receipt for long-term stability. Avoid repeated freeze-thaw cycles, which can degrade protein activity.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimize repeated freeze-thaw cycles.
• Working solution: Keep on ice during experiments and minimize exposure to room temperature.
• Buffer compatibility: Consult the product datasheet for recommended reconstitution buffer and compatible assay buffers.
• Expiry: Follow the expiration date indicated on the product label and certificate of analysis (CoA).

Note: Specific storage conditions, formulation details, and shelf-life data should be confirmed by reviewing the official product datasheet for SKU rp367873 available from Aladdin Scientific.

Ordering and Quality Assurance

Recombinant Human GABA B Receptor 2 Protein is available from Aladdin Scientific under SKU rp367873. When ordering, consider the following:

• Quantity and format: Check the available pack sizes on the Aladdin Scientific product page to select the quantity appropriate for your experimental scale.
• Certificate of Analysis (CoA): A CoA is provided with each lot, documenting purity, concentration, and quality control testing results. Lot-specific CoAs can be requested from the Aladdin Scientific customer service team.
• Quality standards: Aladdin Scientific's recombinant proteins are produced under controlled conditions designed to meet the needs of demanding research applications.
• Custom orders: For large-volume requirements or custom formulations, contact Aladdin Scientific customer support.

For pricing, availability, and bulk order inquiries, please visit the Aladdin Scientific website or contact your regional representative.

Frequently asked questions

What is the biological function of the GABA B Receptor 2 subunit?

The GABA B Receptor 2 (GABBR2) subunit is the G protein-coupling subunit of the heterodimeric GABA B receptor complex. It partners with the GABA B1 subunit to form a functional receptor: while GABA B1 binds the endogenous ligand GABA, GABA B2 couples to inhibitory Gi/o proteins, leading to reduced cAMP levels, modulation of ion channels, and overall inhibition of neuronal excitability. This subunit is therefore essential for transducing the inhibitory GABAergic signal downstream.

What research applications is Recombinant Human GABA B Receptor 2 Protein (rp367873) validated for?

Recombinant Human GABA B Receptor 2 Protein is intended for in vitro research applications including protein–protein interaction studies, biochemical binding assays (such as ELISA and SPR), antibody validation, and drug target screening. Researchers should consult the product datasheet and certificate of analysis for the specific validated applications and assay conditions confirmed for SKU rp367873, as validated uses may vary by lot.

How should Recombinant Human GABA B Receptor 2 Protein be stored to maintain its activity?

As a general best practice for recombinant proteins, this product should be stored at -20°C or -80°C immediately upon receipt to maintain stability. Avoid repeated freeze-thaw cycles by aliquoting the protein into single-use volumes upon first thaw. During experiments, keep the protein on ice and minimize exposure to room temperature. Always refer to the specific storage instructions provided on the product label and datasheet for SKU rp367873.

Can Recombinant Human GABA B Receptor 2 Protein be used for in vivo studies?

Recombinant Human GABA B Receptor 2 Protein (rp367873) is intended for in vitro research use only and is not approved or validated for in vivo administration, diagnostic use, or therapeutic applications. Researchers should strictly adhere to the intended use outlined in the product documentation.

How can I request a Certificate of Analysis (CoA) for lot rp367873?

You can request a lot-specific Certificate of Analysis for Recombinant Human GABA B Receptor 2 Protein (rp367873) by contacting Aladdin Scientific's customer service team directly, either through the website or by reaching out to your regional representative. Provide your lot number (found on the product vial label) when making the request to receive the correct documentation.

References

1. Bettler B, Kaupmann K, Mosbacher J, Gassmann M. Molecular structure and physiological functions of GABA(B) receptors. Physiological Reviews. 2004;84(3):835-867. doi:10.1152/physrev.00036.2003
2. Bowery NG, Bettler B, Froestl W, et al. International Union of Pharmacology. XXXIII. Mammalian gamma-aminobutyric acid(B) receptors: structure and function. Pharmacological Reviews. 2002;54(2):247-264. doi:10.1124/pr.54.2.247
3. Aladdin Scientific. Recombinant Human GABA B Receptor 2 Protein (rp367873) Product Page. Aladdin Scientific website.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.